摘要
应用RAPD技术分析疑肿瘤病人临床肿块穿刺标本。由于微量标本模板DNA量较少 ,采用不同方法抽提DNA明显影响RAPD扩增结果。通过比较热碱法、蛋白酶K/酚法和单管DNA提取方法 ,发现微量穿刺标本用单管法抽提模板DNA优于传统的蛋白酶K/酚法 ,获得的RAPD扩增结果多态性频率高 ,能明显提高实验敏感性。
In this report,the RAPD technique has been applied to analyze the niddle boipsy specimens from different tumor patients.We noted that the measurement errors of RAPD was buffered differentiy with various DNA extraction methods due to limited templates,thus resulted in the distinct profiles of RAPD.We failed to amplify templates obtained by hot base (KOH) treatment.The multiple bands were demonstrated for target DNAs,when proteinase K/phenol extraction or single vial extracting procedure were applied.Superior to phenol extraction,however,using single vial extraction method to prepare template could improve the sensitivity and polymorphisms of RAPD.
出处
《陕西医学检验》
2000年第1期42-44,共3页
Shaanxi Journal of Medical Laboratory Sciences
