摘要
采用快速、敏感和特异性的聚合酶链反应（ＰＣＲ）对１０８名献血员进行了人类巨细胞病毒（ＨＣＭＶ）检测。结果表明，有８４例表现为ＨＣＭＶ阳性，占被检测对象的７７．８％。说明人群中ＨＣＭＶ的感染率是较高的。同时，从本文的ＨＣＭＶ的ＰＣＲ敏感性和待异性试验表明，ＰＣＲ技术可以检测样品中少到２０个拷贝的ＨＣＭＶ模板ＤＮＡ。因此，ＰＣＲ技术是可以替代或弥补目前繁琐的细胞培养技术及血清学方法而用于临床检测的。
amples of peripheral blood from 108 different blooddonors were investigated for the presence of human cy-tomegalovirus(HCMV) DNA with polymerase chain re-action (PCR).The results showed that HCMVs werepresent in 84 out of 108 blood donors,which reached apeak infection rate of 77.8%.In addition, the test ofspecificity and sensitivity on PCR showed the surtabilityof the PCR as a potential diagnostic tool,which mightsubstitute or compensate for routine methods.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第5期254-255,共2页
Chinese Journal of Hematology
关键词
巨细胞病毒
输血
聚合酶链反应
Human cytomegalovirus TransfusionPolymerase chain reaction
