摘要
建立高效液相色谱法同时测定丹参植株中不同部位丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B五种水溶性成分的含量。采用DiamonsilTM C18色谱柱(250mm×4.6mm,5μm),以5%冰醋酸和甲醇为流动相进行梯度洗脱,柱温:35℃,流速为1.0mL/min,检测波长为288nm。丹参素、原儿茶酸、原儿茶醛、迷迭香酸和丹酚酸B的线性范围分别为0.35-21μg.mL-1(r=0.9999),0.3625-21.75μg.mL-1(r=0.9997),0.32-19.2μg.mL-1(r=0.9999),1.92-115.2μg.mL-1(r=0.9998),8.1-483.6μg.mL-1(r=0.9998)。平均回收率丹参素为99.3%(RSD 1.5%),原儿茶酸为99.7%(RSD 1.1%),原儿茶醛为99.8%(RSD1.7%),迷迭香酸为100.1%(RSD 0.4%),丹酚酸B为99.7%(RSD 1.5%)。本方法快速简便,5组分分离良好,检测结果为综合开发利用丹参地上部分资源提供科学依据。
To establish an HPLC with diode array detection for simultaneous determination of five watersoluble compounds： danshensu, protocatechuic aicd, pmtocatechuic aldehyde, rosmarinic acid and sal-vianic acid B in different part of Danshen. The column was Diamonsil^TM C18 （250mm×4.6mm,5um）. The mobile phase was methanol and 5% acetic acid glacial with gradient elution and the column temperature was 35℃, the flow rate was 1.0mL/min, the detection wavelength was set up at 288nm. The linearity was obtained over the ranges of 0.35-21ug.mL-1 （r=0.9999） for danshensu with average recovery of 99.3%（RSD was 1.5%）, 0.36-21.75ug.mL-1 （r=0.9997） for protocatechuic aicd with average recovery of 99.3%（RSD was 1.5%）, 0.32-19.2ug.mL-1 （r=0.9999） for protocatechuic aldehyde with average recovery of 99.8% （RSD wasl.7%）, 1.92-115.2ug.mL-1（r= 0.9998） for rosmarinic acid with average recovery of 100.1% （RSD was 0.4%） 8.1-483.6ug.mL-1 （r=0.9998） for sal-vianic acid B with average recovery of 99.7% （RSD wasl.5%）. The method is rapid, simple, and reliable, and can be used for simultaneous determination of five watersoluble compounds in different part of Danshen.
出处
《食品与发酵科技》
CAS
2012年第3期86-89,共4页
Food and Fermentation Science & Technology
基金
四川省科技厅科技支撑项目(2010SZ0242)
四川省教育厅大学生创新性实验项目(Sc-mnu1115)
关键词
中江丹参
有效成分
高效液相色谱法
资源利用
salvia mihiorrhiza of Zhong jiang
effective component
HPLC
resource utilization
