摘要
目的研究肿瘤坏死因子基因TNF-α-308、TNF-α-238与广西肝癌家族聚集性的关系。方法在广西肝癌高发区选取12个肝癌高发家族(124例)及与之相对应的12个非肿瘤对照家族(129例)为研究对象,采用SNP芯片技术检测两组中TNF-α-308、TNF-α-238的基因型及等位基因频率。结果肝癌高发家族组TNF-α-308G/A基因型分布频率高于对照家族组,差异有统计学意义(22.6%vs11.6%,χ2=5.376,P=0.020);肝癌高发家族组TNF-α-308A等位基因分布频率亦高于对照家族组,差异有统计学意义(11.3%vs5.8%,χ2=4.877,P=0.024)。两组TNF-α-238基因型频率及等位基因频率差异均无统计学意义(P>0.05)。结论 TNF-α-308基因多态性可能与广西肝癌家族聚集性相关,携带TNF-α-308A等位基因可能会增加肝癌高发家族成员患肝癌的风险;TNF-α-238基因多态性与广西肝癌家族聚集无统计学关联。
Objective To explore the correlation between genetic polymorphisms of tumor necrosis factor （TNF-a-308,TNF-a-238） and family clustering of primary liver cancer（PLC ） in Guangxi. Methods Blood samples were collected from 124 families with a high incidence of PLC and from 129 control families.Genotype and alleles of TNF-a-308 and TNF-a-238 were determined by SNP microar- ray technology. Results The frequencies of TNF-a-308G/A genotype and TNF-a-308A allele in the PLC-clustering families （22.6% and 11.3% ）were significantly higher than those in the control families （11.6% and 5.8% ）.However,the two groups showed no statisti-cally significant differences in frequencies of the TNF-a-238 genotype or allele（P〉0.05）. Conclusions TNF-a-308 genetic polymorph-ism is associated with susceptibility in families with high PLC incidence in Guangxi. In particular,the TNF-a-308A allele is proba-bly a risk factor for PLC in these families. In contrast,the TNF-a-238 polymorphism may not be associated with family clustering of PLC in Guangxi.
出处
《中国癌症防治杂志》
CAS
2012年第2期149-153,共5页
CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT
基金
国家自然科学基金资助项目(30960333)
广西自然科学基金资助项目(桂科自099107Z)
广西卫生厅医疗卫生重点科研课题(重2010117)
广西卫生厅自筹经费科研课题(Z2007175)
广西研究生教育创新计划资助项目(2011105981002M176)
