摘要
目的探讨子宫内膜癌组织肿瘤坏死因子（ＴＮＦ）基因和蛋白及受体的表达及意义。②方法分别采用非同位素原位杂交方法、免疫组化方法对４８例子宫内膜癌组织ＴＮＦｍＲＮＡ，ＴＮＦ蛋白及ＴＮＦ受体（ＴＮＦＲ）的表达进行检测。③结果子宫内膜癌组织中１６例ＴＮＦｍＲＮＡ呈阳性表达，均为间质的单个核细胞和平滑肌细胞，腺癌细胞无阳性表达，对照组未见阳性表达。１５例病人ＴＮＦ蛋白呈阳性表达，２５例ＴＮＦＲ呈阳性表达，对照组子宫内膜腺体也有ＴＮＦＲ阳性表达，两组比较差异无显著性（χ２＝２．４５，Ｐ＞０．０５）。ＴＮＦｍＲＮＡ与ＴＮＦ蛋白表达存在不一致性，ＴＮＦ与ＴＮＦＲ表达与组织学分化及肌层浸润深度差异均无显著性（χ２＝２．１７，０．８１，Ｐ＞０．０５）；ＴＮＦＲ蛋白表达明显高于ＴＮＦ，两者比较差异有极显著性（χ２＝４．２９，Ｐ＜０．０５）；ＴＮＦＲ在正常内膜表达与子宫内膜癌表达差异无显著性（χ２＝２．４５，Ｐ＞０．０５）。④结论子宫内膜癌间质细胞能够表达ＴＮＦｍＲＮＡ，并产生ＴＮＦ蛋白。
\ Objective \ To observe the
expressions and distributions of tumor necrosis factor (TNF) mRNA, TNF protien and TNF
receptor(TNFR) in endometrial carcinomas and investigate their clinicopathological significance.
\ Methods \ The expressions of TNF mRNA, TNF protein and TNFR in 48 cases of endometrial
carcinoma were studied by in situ hybridization and immunohistochemical techniques.\
Results \ TNF mRNA expression was detected in the stroma such as mononuclear cells and
smooth muscles in 16 cases of endometrial carcinoma. There was no TNF mRNA expression in
the carcinoma cells and controls. TNF protein was positive in 15 cases of endometrial
carcinoma. TNFR expression was detected both in endometrial carcinomas(25 cases) and
controls(18 cases), the differences was not significant (2=2.45, P>0.05). The expressions of TNF
and TNFR in cases of different histologic grades and depth of invasion were not significantly
different(2=2.17, P>0.05). TNFR expression in endometrial carcinoma was higher than that of
TNF(2=4.29, P<0.05). The expression of TNFR in normal endometrium and endometrial
carcinoma was not significantly defferent(2=2.45, P>0.05).\ Conclusion \ The stroma cells in
endometrial carcinoma were capable of expressing TNF mRNA and producing TNF
protein.
出处
《青岛医学院学报》
1999年第1期10-12,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
卫生部科研基金
关键词
肿瘤坏死因子
子宫肿瘤
受体
原位杂交
\
\ tumor necrosis factor
endometrial carcinoma
hybridization
immunohistochemistry
