摘要
目的:观察石见穿对肝癌H22小鼠肿瘤生长的影响,并探讨其可能的作用机制。方法:肝癌H22荷瘤小鼠随机分为5组:模型组、石见穿提取物高、中、低(40,10,2.5 g.kg-1ig)3个剂量组和阳性药环磷酰胺(CTX,0.025 g.kg-1,ip)对照组。连续10 d。于第11天观测抑瘤率、脾指数、胸腺指数、血清中肿瘤坏死因子-α(TNF-α)和血骨内皮细胞生长因子(VEGF)的含量。结果:与模型组比较,石见穿提取物高、中、低剂量组的抑瘤率分别为23.83%,48.93%(P<0.01)和27.48(P<0.05);石见穿提取物中、低剂量组脾指数和胸腺指数升高,但差异无统计学意义;石见穿提取物各剂量组血清中TNF-α和VEGF含量下降,以中剂量组VEGF含量下降明显,模型组(28.64±7.18)pg.mL-1,中剂量组(21.22±5.60)pg.mL-1(P<0.05)。结论:石见穿提取物对肝癌H22荷瘤小鼠肿瘤的生长具有明显的抑制作用,其作用机制可能与下调细胞因子VEGF含量有关。
Objective： To evaluate the inhibitory effect of extracts from Salvia chinensis against H22 tumor in mice, and analyze the mechanism of inhibiting the growth of tumor. Method： H22 tumor-bearing mice were randomly divided into five groups, they were control group, high, middle and low dosages of extracts from S. chinensis, and positive-control group. All the treatment lasted 10 days. We investigated the inhibition rate, spleen index, thymus index, content of tumor necrosis factor α （TNF-α） and vascular endothelial cell growth factor （VEGF） in serum at the 1 1^th day. Result： In contrast to model group, the inhibition rate of the high, middle, and low dosages of S. chinensis extracts were 23.83% , 48.93% （P 〈 0.01 ） and 27.48 （ P 〈 0.05 ） , respectively. The spleen index and thymus index of middle and low dosages of S. chinensis extracts rose, but there was not statistical significance. The content of TNF-α and VEGF in serum of administered group were decreased, and the middle dosage of S. chinensis was remarkable （P 〈 0.05 ）. Conclusion： S. chinensis benth extracts could obviously inhibit tumor growth of H22 in tumor-bearing mice, and maybe it involved the down regulation of VEGF.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期249-251,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家重大新药创制专项(2009ZX09301-005-007)
关键词
石见穿
肿瘤
肝癌H22
免疫
Salvia chinensis
tumor
hepatoma H22
immune
