摘要
目的:应用单细胞凝胶电泳技术评价牛大力的遗传毒性。方法:40只小鼠随机分成5组:阳性对照组(环磷酰胺组)、阴性对照组(生理盐水组)、牛大力高、中、低(20,10,5 g.kg-1)剂量组。牛大力各剂量组用牛大力煎煮液ig,阴性对照组、阳性对照组ig等量生理盐水,每天1次,连续10 d,阳性对照组在最后2 d ip环磷酰胺(0.08 g.kg-1),每天1次,在最后1次给药6 h后,处死各组小鼠,取肺、肾、肝、睾丸细胞,利用单细胞凝胶电泳技术观察牛大力对小鼠肺、肾、肝、睾丸细胞DNA的影响。结果:牛大力各剂量组对小鼠肺、肾、肝、睾丸细胞的尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)的影响明显低于阳性对照组,差异有统计学意义(P<0.01),牛大力高剂量组肾细胞Tail DNA%与阴性对照组比明显降低,统计学上有显著差异(P<0.05),其他各剂量组小鼠细胞tail DNA%和tail moment与阴性对照组比,差异无统计学意义。结论:牛大力对小鼠肺、肾、肝、睾丸细胞DNA未观察到明显损伤。
Objective： To evaluate genotoxicity of Radix Millettiae Speciosae by single cell gel electrophoresis. Method： Forty mice were divided into five groups randomly： negative control group, positive control group, and Radix Millettiae Speciosae group with high （20 g·kg^-1）, middle （10 g·kg^-1） or low （5 g·kg^-1 ） doses. Radix Millettiae Speciosae groups were given with relative drugs, Negative control group and positive control group were given with normal saline by gavage once a day for 10 days, while the last two days, positive control group was injected with cyclophosphamide （0.08 g·kg^-1 ） once a day. In the eleventh day, the mice were sacreficed, DNA damage of liver, kidney, lung, testicular ceils was detected by single cell gel electrophoresis. Result： Compared with positive control group, tail DNA% and tail moment of liver, kidney, lung, testicular ceils in Radix Millettiae Speciosae group had a significant lowereduction （P 〈 0.01 ）. Compared with the negative control group, tail DNA% and tail moment of kidney cells in the Millettiae Speciosae group with high dose showed a significant reduction （P 〈 0.05） , tail moment and tail DNA% of ther cells in other dose group, no statistically significant difference was found. Conclusion： Radix Millettia Speciosa has no damage to mouse cells DNA.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期222-225,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西自然科学基金面上项目(2011GXNSFA018296)
关键词
牛大力
单细胞凝胶电泳
遗传毒性
Radix Millettiae Speciosae
single cell gel electrophoresis
genotoxicity
