摘要
目的:建立绞股蓝药材黄酮类成分HPLC指纹图谱。方法:采用反相高效液相色谱法,色谱柱:TURNERKromasilTMC18色谱柱(250×4.6 mm,5μm);流动相:0.4%磷酸水溶液-甲醇-乙腈(梯度洗脱);流速:0.5 ml/min;检测波长:360 nm。结果:建立了绞股蓝药材黄酮类成分HPLC指纹图谱,标定了10个共有指纹特征峰,方法学考察符合指纹图谱技术要求。结论:方法稳定,可靠,精密度高,重复性好,可为绞股蓝药材质量标准的制定提供科学依据。
Objective： To establish the HPLC fingerprint spectrum of flavonoids from Gynostemma pentaphyllum. Methods： The HPLC conditions with TURNER Kromasil^TM C 18 column（250×4. 6 mm,5 μm） was used, the mobile phase consisted of 0.4% phosphoric acid-methanol-acetonitrile with gradient elution at flow rate of 0. 5ml/min and the detection wavelength was set at 360nm. Results： Ten common characteristic peaks were taken as fingerprint peaks the presision and the retest are in accordance with the require- ment. Conclusion： The method is stable, reliable, precision and provides a scientific basis for the quality standard of Cynostemma pentaphyllum.
出处
《中药材》
CAS
CSCD
北大核心
2007年第8期929-932,共4页
Journal of Chinese Medicinal Materials
基金
十五国家重大科技专项课题"创新药物和中药现代化"(2001BA701A36-17)
关键词
绞股蓝
黄酮类成分
HPLC
指纹图谱
Gynostemma pentaphyllum
Flavonoids
HPLC
Fingerprint Spectrum
