摘要
目的:建立金莲花中金莲花苷的含量测定方法,并测定其在市售金莲花药材中的含量。方法:高效液相色谱法,Kromasil C18ODS色谱柱(4.6 mm×250 mm,5μm),柱温为室温;流动相A为乙腈,B为0.5%醋酸溶液;洗脱程序(0～30min,2%～100%A),流速1.0 mL.min-1,检测波长258 nm。结果:金莲花苷在0.115 8～1.042 2μg线性关系良好,9批金莲花药材中的金莲花苷的含量为0～0.058%。结论:本方法精密度、准确度和重复性良好,适用于测定金莲花中金莲花苷的含量。
Objective： To establish a HPLC assay method for trollioside and determine its content in the flowers Of Trollius chinensis commercially available in China. Method： High performance liquid chromatography was employed using kromasil Cls ODS column （4.6 mm× 250 mm, 5 μm）, and mobile phase comprising acetonitrile-0.5% acetic acid solution （gradient： 0-30 rain, 2%-100% A） at a flow rate of 1.0 mL.min-1 and detecting wavelength of 258 nm. Result： A good linear relationship was demonstrated for trollioside within 0. 115 8- 1. 042 2 μg and the content of trollioside in nine batches of the flowers of T. chinensis commercially available in China was within 0-0.058%. Conclusion： This assay method is good in terms of precision, accuracy and repeatability and can be used for determination of the content of trollioside.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第12期75-77,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81073018)
教育部留学回国启动金项目
