摘要
本实验选用家兔,定量研究脑损伤早期血-脑屏障(BBB)通透性变化。并检测脑组织水含量变化。结果表明:BBB通透性在伤后0.5h已开始增高,伤后6h,通透性增高达高峰,并伴有脑组织水含量明显增加,导致血管源性脑水肿。山莨菪碱可显著降低BBB通透性,并有利于脑水肿的防治。
Fifty-seven rabbits were used for the experimental study of blood-brain barrier (BBB)permeability quantitatively in early stage of brain injury by a designated drop- ping weight method.Animals were divided into three groups:(1) control;(2)brain injury group; (3)therapeutic group.The brain injured animals were treated with antisodamine.Three different sizes of colloidal gold (OG)particles in diameter 5,10 and 15mu were given intravenously as tracers for quantitative investigation of BBB changes with light and electron microscope,respectively.In addition,brain water contents were de4rmined,The preliminary data indicated that the increase of BBB permeability began at 0.5h after trauma showing a few of 5 and 10 mu CG particles present in the endocytic pits and endothelial microvilli.Augmentation of BBB per meability reached its peak 6h after injury.Many 5,10 and 15mu CG tracers were seen penetrating the BBB through the openings at the tight junctions of endothelial cells or by vesicular transportation.The variation of brain water contents was closely correlated to the above mentioned BBB changes.The use of antisodamine resulted in a remarkable attanuation of BBB permeability and seemed advantageous in the treat- meat of cerebral edema after brain injury.
出处
《中华创伤杂志》
CAS
CSCD
北大核心
1990年第3期142-144,共3页
Chinese Journal of Trauma
关键词
脑损伤
脑水肿
血脑异障
通透性
blood-brain barrier
brain edema
brain injunies
colloids
