摘要
目的通过检测WIF-1与Wnt2b在正常大肠黏膜、大肠腺瘤、大肠癌中的表达及其与大肠癌生成、侵袭转移之间的关系,探讨WIF-1与Wnt2b之间的关系。方法应用免疫组化SP法检测WIF-1和Wnt2b蛋白在15例癌旁正常组织、18例大肠腺瘤和103例大肠癌组织中的表达。结果 (1)WIF-1蛋白在大肠癌中的阳性率明显低于癌旁正常组织和腺瘤;Wnt2b蛋白在大肠癌中的阳性率高于癌旁正常组织和腺瘤。(2)WIF-1在大肠癌中的表达与患者的年龄、性别、分化程度、浸润程度、淋巴转移和远处转移均无关系(P>0.05);Wnt2b在大肠癌中的表达与大肠癌的分化程度、Dukes分期、淋巴结转移和远处器官转移相关(P<0.05),而与患者的年龄、性别均无关(P>0.05)。(3)大肠癌中WIF-1与Wnt2b的表达呈负相关(r=-0.199,P<0.05)。结论 WIF-1在正常大肠组织中呈阳性表达,在大肠癌的发病机制中可能发挥一定作用;Wnt2b的表达与大肠癌的发生、侵袭转移有关,联合检测WIF-1和Wnt2b有助于大肠癌的诊断、预防和治疗。
Purpose To study the expression of wnt inhibitor factor-1 （ WIF-1 ） and Wnt2b in human adjacent normal colorectal muco- sa, adenomas and eoloreetal carcinoma with invasion and metastasis, and to explore the correlation between WIF-1 and Wnt2b. Meth- ods The expression of WIF-1 and Wnt2b was detected by immunhistochemical staining （ streptavidine-peroxidase method） in adjacent normal coloreetal mucosa, adenomas and eoloreetal carcinoma. Results （ 1 ） The expression rate of WIF-1 in eolorectal carcinoma was significantly lower than that in adjacent normal coloreetal mucosa and adenomas, and that of Wnt2b in eolorectal carcinoma was significantly higher than that in adjacent normal colorectal mucosa and adenomas. （2） The expression of WIF-1 in colorectal carcinoma did not correlate with the patients, age, gender, tumor size, Dukes stages, lymph node status and metastasis （P 〉 0. 05）. The expres- sion of Wnt2b in eoloreetal carcinoma did not correlate with the patients＇ age, gender （ P 〉 0. 05 ） , but correlated with tumor size, Dukes stages, lymph node status and metastasis （P 〈 0. 05 ）. （ 3 ） The expression of WIF-1 and Wnt2b was negatively related in the colorectal carcinoma （ r = - 0. 199, P 〈 0. 05 ）. Conclusion WIF-1 express periodically in normal colorectal, and may play a role in the pathogenesis of colorectal cancer, and the expression of Wnt2b is correlated with tumorigenesis and invasion and metastasis. Com- bined detection of WIF-1 and Wnt2b can serve as a parametar for diagnosis of colorectal carcinoma and help for its prevention and treat- ment.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2012年第6期613-615,619,共4页
Chinese Journal of Clinical and Experimental Pathology
