摘要
利用F0F1-ATP合酶的旋转特性及对H+敏感的荧光物质F-DHPE作为指示剂来检测样本中有无目标物。通过生物素-亲和素系统将特异性invA核酸探针连接在F0F1-ATPase的ε亚基上构建生物传感器;将待测样品和阴性对照分别与生物传感器结合后,比较其催化ATP合成30min后的ATP产生量,依此对样品中的沙门氏菌DNA进行检测。结果表明,该方法对沙门氏菌DNA的检测时间为1h,检出限为10ng/mL。从食品样本分离得到的30株细菌,利用分子马达生物传感器的检测结果与PCR检测的结果一致。
Using F0F1-ATPase of spin characteristics and H＋-sensitive fluorescent material F-DHPE as an indicator to detect the target sample.Specific invA probe were connected with F0F1-ATPase’s ε subunit by using avidin-biotin system,and then biosensors were constructed,the test sample and negative sample,respectively,combined with biosensors,to compare their catalytic ATP synthesis after 30min,Salmonella DNA in the samples can be tested.According to the result of our experiment,it took 1h and could detect accurately about 10ng/mL for standard strain.30 strains of food sample which were detected with the PCR detection results that were consistent.
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第12期93-96,共4页
Science and Technology of Food Industry
基金
国家质检总局科技计划项目(2008IK161)
