摘要
目的用酶免疫吸附试验(EIA)法检测北京地区婴幼儿腹泻标本中的Noro病毒,试图找到一种简便易行的检测方法,以期应用于临床检测。方法2003年10月至2004年12月在首都儿科研究所附属儿童医院就诊的腹泻患儿粪便标本中抽取167份应用EIA进行Noro病毒抗原检测,其中有61份同时进行聚丙烯酰胺凝胶电泳检测轮状病毒。结果167份粪便标本中,Noro病毒阳性46份,阳性率为27.5%。其中,GⅠ型阳性标本20份,占阳性标本总数的43.5%(20/46);GⅡ型阳性标本19份,占阳性标本总数的41.3%(19/46);另有7份标本为GⅠ和GⅡ型同时阳性,提示为混合感染,占阳性标本总数的15.2%(7/46)。发病年龄以2岁以下患儿为主,占总感染人数的93.5%(43/46)。本组Noro病毒感染性腹泻未见明显的季节性规律。在检测轮状病毒的61份标本中,轮状病毒阳性29份,阳性率为47.5%。在29份轮状病毒阳性标本中,11份(11/61,18.0%)标本检出Noro病毒阳性,提示存在Noro病毒和轮状病毒的混合感染。结论Noro病毒感染在北京地区婴幼儿腹泻中占有重要地位,仅次于轮状病毒。EIA检测Noro病毒简便易行、利于推广。
Human Noro virus is one of the most important etiological agents of non-bacterial diarrheal illness of infants and young children, especially in developing countries. The complex and variable molecular epidemiology of human Noro viruses infection make it difficult to be detected. The method having been used to detect human Noro virus is reverse transcription-polymerase chain reaction （RT-PCR）, which takes long time and can not be performed in all laboratories. Objective To detect human Noro virus infections in specimens collected from infants and young children with acute non-bacterial diarrhea in Beijing by enzyme immunoassay （EIA） and to find a more convenient method to detect human Noro viruses rapidly and efficiently. Methods Enzyme immunoassay （EIA） was used to detect human Noro viruses in stool specimens collected from infants and young children with acute non-bacterial diarrhea who visited the affiliated Children＇s Hospital to Capital Institute of Pediatrics from Oct 2003 to Dec 2004. Some of these specimens were tested for rotavirus by poly-acrylamide gel electrophoresis （PAGE）. The data were statistically analyzed by using SPSS 11.5 software. The χ^2 test was used to test categorical variables. All tests were considered to be significant when P 〈0.05. Results Out of 167 stool specimens collected from infants and young children with acute non-bacterial diarrhea in the affiliated childreng hospital to Capital Institute of Pediatrics from October 2003 to December 2004, 46 （ 27.54% ） were Noro virus positive as determined by EIA. Among those 46 positive specimens, 20 （43.48%） were Noro virus G Ⅰ positive and 19 （41.30%） were Noro virus G Ⅱ positive and 7 （ 15.22% ） were both Noro virus G Ⅰ and G Ⅱ positive . Most of the Noro viruses positive specimens were those collected from children younger than 2 years old. The youngest infant with positive specimens was 2 months old and the oldest child was 7 years old in this study. Among those 46 positive specimens, 12 （26.1% ） were obtained from infants younger than 6 months old, 22 （47.8%） from those aged 7 - 12 months, 6 （ 13.0% ） from the age group of 1 to 1 year and 6months old, 3 （6.5%） from the age group of 1 year and 6 months to 2 years old, and 3 （6.5%） were from children older than 2 years old. The χ^2 test used to test categorical variables showed P = 0. 319 （ P 〉 0. 05 ） , suggested no seasonal cluster was found. In those 167 stool specimens, 61 were chosen to detect Rotavirus by poly-acrylamide gel electrophoresis, and 29 （47.54%） were positive for rotavirus. 18 （29.5%） showed positive reaction for ro/avirus, but negative for human Noro viruses. And 11 （ 18.0% ） of the specimens detected showed positive reactions for both Noro virus and Rotavirus, suggested that these children were coinfected with two viruses. Conclusions Human Noro virus is one of the most important pathogens for non-bacterial diarrhea among infants and young children in Beijing. There were some co-infection with human Noro viruses and rotavirus in infants and young children in Beijing. Using EIA to detect human Noro viruses infection is more convenient and time-saving than RT-PCR. The epidemiology of human Noro viruses in Beijing needs to be determined.
出处
《中国循证儿科杂志》
CSCD
2006年第3期199-203,共5页
Chinese Journal of Evidence Based Pediatrics
基金
国家自然科学基金(30270067)
关键词
非细菌性腹泻
Nora病毒
轮状病毒
婴幼儿腹泻
酶免疫吸附法
Non-bacterial diarrhea
Noro virus
Rotavirus
Infants and young children with acute diarrhea
Enzyme immunoassay
