摘要
【目的】建立一种简单有效的产苯乳酸乳酸菌的筛选方法,并筛选到高产苯乳酸的乳酸菌。【方法】菌株经过添加了苯丙氨酸的培养基厌氧培养后,利用高效液相色谱法检测发酵液中苯乳酸的含量。【结果】从猪的消化道中共分离得到31株产苯乳酸的乳酸菌,其中菌株R53的苯乳酸产量最大,为321.7 mg/L。菌株R53对.OH、O2和DPPH都有清除能力,清除率分别达到11.2%、52.7%和63.2%。同时R53也能降低培养基中胆固醇的含量,清除率为32.5%。【结论】乳酸菌R53经菌落形态、细胞形态、生化反应实验、16S rDNA测序,最终确定为植物乳杆菌。植物乳杆菌R53能产生苯乳酸,具有清除胆固醇和抗氧化能力。
[Objective] A simple and efficient method for screening lactic acid bacteria which would produce phenyllactic acid （PLA） would be developed. [Methods] Strain R53 was incu- bated in MRS （ de Man, Rogosa, Sharpe ） broth supplemented with phenylalanine by the an- aerobic culture, and PLA in the fermentation broth was determined by HPLC. [Results] 31 strains were obtained from swine＇s digestive tract, of which strain R53 from duodenum showed the highest PLA-producing ability （321.7 mg/L）. Strain R53 showed the ability to inhibit the on the three radicals. The results showed that the radical-scavenging ratios on -OH, 02- ,andDPPH were 11.2%, 52.7% and 63.2% respectively. In addition, strain R53 could degrade cho- lesterol and the degradation rate was 32.5% in vitro. [Conclusion] Strain R53 was identified as Lactobacillus plantarum through its culture characteristics, morphological observation, bio- chemistry experiment and combining with the phylogenetic analysis of 16S rDNA. L. plantarum R53 had the ability of phenyllactic acid-producing, cholesterol-reducing, and free radical-scavenging.
出处
《微生物学通报》
CAS
CSCD
北大核心
2012年第6期804-810,共7页
Microbiology China
基金
国家自然科学基金项目(No.30671398
31070005)
关键词
猪
乳酸菌
苯乳酸
抗氧化作用
降胆固醇
Swine, Lactic acid bacteria, Phenyllactic acid, Antioxidative effect, Cholesterol removal
