摘要
通过单因素试验分别研究温度和IPTG浓度对重组大肠杆菌BL21(DE3)/pProEXHTa-PIUGT2诱导表达野葛糖基转移酶PlUGT2蛋白量的影响。利用150 ml LB液体培养基培养重组大肠杆菌BL21(DE3),并优化发酵条件。结果表明,在温度20℃和IPTG浓度为0.75 mmol/L条件下,PIUGT2蛋白表达量最高。
Based on the single factor of experiment, the effects of temperature and IPTG concentration on BL21（DE3）/pProEXHTa-PIUGT2 protein production were studied. Fermentation conditions of BL21（DE3）/pProEXHTa-PIUGT2 cultured in 150 ml LB medium were optimized. The expression conditions of P1UGT2 protein was high when culture condition to be 0.75% of IPTG concentration and 20℃.
出处
《亚热带植物科学》
2012年第2期9-12,共4页
Subtropical Plant Science
基金
广东省科技计划项目(2009B020301003)
关键词
原核表达
温度
IPTG浓度
prokaryotic expression
temperature
IPTG concentration
