摘要
通过生物信息学和同源建模分析发现甘薯mPPO含有588个氨基酸残基(aa),其N-端包含两段跨膜信号序列.二级结构预测结果显示,mPPO含有18个α-螺旋、9个β-转角、18个无规则卷曲,同时二硫键稳定着酶分子结构,活性中心被6个组氨酸包围.分子对接结果揭示了mPPO与底物的结合位点以及相互作用方式.
In a present study,bioinformatics and homology modeling analysis showed that mPPO of sweet potato contained 588 amino acid residues,and its N-terminal contained two transmembrane signal sequences;secondary structure prediction results indicated that mPPO were composed of 18α-helices,9β-turns and 18 random coils,disulfide bonds stabilized the molecular structure of the enzyme,and the active center was surrounded by 6 molecules;and molecular docking results revealed the binding sites and the interaction modes between mPPO and its substrates.
作者
李丰茂
杨浩
傅玉凡
陈晓玲
唐云明
LI Feng-mao;YANG Hao;FU Yu-fan;CHEN Xiao-ling;TANG Yun-ming(School of Life Science,Southwest University/Engineering&Technology Center for Sweet Potato,Chongqing 400715,China;Tongnan High School,Tongnan Chongqing 402660,China;Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
出处
《西南大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第6期60-66,共7页
Journal of Southwest University(Natural Science Edition)
基金
财政部和农业农村部国家现代农业产业技术体系建设专项(CARS-10-C20-2020)
农业农村部农作物种质资源保护与利用专项(2018NWB036-09-01).
关键词
甘薯
膜结合态多酚氧化酶
褐变
同源建模
分子对接
sweet potato
membrane-bound polyphenol oxidase
browning
homology modeling
molecular docking
