摘要
目的观察不同浓度地黄对肾脏组织抗氧化作用的影响,探讨地黄在治疗肾病时的适宜剂量。方法将患者分为6组,其中空白组不给任何药物,模型对照组单纯造模而不给药物,阳性对照组给予维生素E,高、中、低浓度给药组(分别给予地黄6.5 mg/ml,3.25 mg/ml和1.625 mg/ml)。分别测定各组处理后H2O2诱导的大鼠肾匀浆脂质过氧化、大鼠肾匀浆产生自发性脂质过氧化产物丙二醛(MDA)的含量以计算MDA抑制率,及对诱导红细胞溶血的溶血率。结果阳性对照组、高、中、低浓度给药组MDA抑制率均较模型对照组高(P均<0.05),而高、低浓度给药组MDA抑制率较中浓度给药组低(P均<0.05),且中浓度给药组MDA抑制率与阳性对照组无统计学差异(P>0.05);阳性对照组、高、中、低浓度给药组溶血率均较模型组低(P<0.05),而高、低浓度给药组溶血率较中浓度给药组高(P均<0.05),且中浓度给药组溶血率与阳性对照组无统计学差异(P>0.05)。结论不同浓度的地黄均有明显的抗氧化作用,且抗氧化作用与剂量相关。在治疗肾脏疾病的复方配伍中,地黄宜用中等量。
Objective To investigate the antioxidation ability of tissue induced by various doses of rehmannia in vitro for getting the optimal dose of rehmannia. Methods All the patients were divided into three groups,blank control group, model control group（ gave no drug but only modeling）, positive control group （gave vitamin E）, and low, median and high dose groups （gave rehmannia 6.5 mg/mL, 3.25 mg/mL and 1. 625 mg/mL respectively）. The concentration of malondialdehyde （MDA） in hydrogen peroxide-induced and spontaneous lipid peroxidation of renal tissue homogenate, and the inhibition ratio of hemolysis induced by hydrogen peroxide were measured. Results The MDA inhibition ratios elevated significantly in positive control group and low, median and high dose groups compared with the model control group group （P 〈 0.05 ）. The MDA inhibition ratios of low and high dose groups were lower than that of median dose group （ P 〈 0.05 ）, but similar with median dose group and positive control group （ P 〉 0.05 ）. Compared with group of model group, the MDA inhibition ratio decreased in others groups （ P 〈 0.05 ）. The hemolysis ratio of model control group was lower than those of low, median and high dose groups （P 〈 0.05 ）, the hemolysis ratio in median dose group was higher than those of low and high dose groups （P 〈 0.05 ）, but similar with the positive control group （ P 〉 0.05 ）. Conclusions Various doses of rehmannia had the antioxidation abilities, which showed a dose-dependence effect. In the compound preparation of drug used in renal diseases, the doses of rehmannia should be moderate.
出处
《内科》
2012年第3期220-223,共4页
Internal Medicine
基金
广西中医药民族医药科研课题(gzzc1131)
关键词
地黄
抗氧化
肾组织
大鼠
丙二醛
红细胞溶血
Rehmannia
Atioxidation
Renal tissue
Rat
Malondialdehyde
Red blood cells hemolysis
