摘要
[目的]研究加味桃核承气汤对糖尿病大鼠的肝、肾和心脏组织抗氧化保护作用。[方法]尾静脉小剂量(45 mg/kg)注射链脲佐菌素(STZ)复制糖尿病模型,设立对照组、糖尿病组和给药组(加味桃核承气汤10 ml/mg·d),测定第56天各组的左肾指数、右肾指数和肝指数,测定肝、肾和心组织匀浆中的过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。[结果]给药组大鼠的体重较糖尿病组极显著升高(P<0.01),左肾指数和右肾指数较糖尿病组无显著变化,肝指数较糖尿病组极显著降低(P<0.01);给药组的肝脏中的SOD和CAT均显著高于糖尿病组(P<0.05);心脏中的GSH-Px显著高于糖尿病组(P<0.05);给药组肝、肾和心脏中MDA含量均显著低于糖尿病组(P<0.05)。[结论]加味桃核承气汤可通过清除氧自由基及抗脂质过氧化作用,提高糖尿病大鼠的肝、肾和心组织的抗氧化能力,缓解氧化损伤。
[Objective] To study the anti-oxidative activity of Taohe Chengqi Decoction on liver,kidney and heart of diabetic rats.[Method] Diabetic models were established by tail vein injection of small dose streptozotocin （45 mg/kg）,the three groups divided：control group,diabetes group,treatment group（Taohe Chengqi decoction 10 ml/mg · d）.At the 56 th day,left kidney index,right kidney index and liver index were detected,the levels of CAT,MDA,GSH-Px and SOD in liver,kidney and heart homogenate were detected.[Result] The body weight of treatment group were significantly higher with diabetes group （P 〈 0.01）,left kidney index and right kidney index of diabetes group were no significant change,liver index very significantly reduced compared with diabetes group（P 〈 0.01） ; SOD and CAT activities in liver were significantly higher in treatment group than those in diabetes group（P 〈 0.05） ; GSH-Px activities in heart were significantly higher than diabetes group （P 〈 0.05） ;level of MDA were significantly lower in liver,kidney and heart tissue in treatment group than diabetes group （P 〈 0.05）.[Conclusion] Taohe Chengqi Decoction could improve diabetic rat liver,kidney and heart tissue antioxidant capacity,reduce oxidative damage through oxygen free radical scavenging and anti-lipid peroxidation.
出处
《安徽农业科学》
CAS
2013年第30期12052-12054,12058,共4页
Journal of Anhui Agricultural Sciences
基金
安徽科技学院引进人才专项基金资助项目(ZRC2007139)
安徽科技学院自然科学研究项目(ZRC2013366)
安徽科技学院重点建设课程项目(ZDKC1130)
关键词
糖尿病
桃核承气汤
链脲佐菌素
抗氧化
Diabetic mellitus
Taohe Chengqi Decoction
Streptozotocin
Antioxidative
