摘要
γ-聚谷氨酸是由微生物分泌的水溶性生物高分子化合物,因其具有无毒可降解特点,而成为备受瞩目的新型高分子材料,具有广泛地应用前景。该文采用大豆培养液发酵法制备并分离纯化了γ-聚谷氨酸;用薄层层析鉴定γ-聚谷氨酸水解产物;采用全波长扫描分析光谱学特性并用紫外分光光度法测定γ-聚谷氨酸浓度;SDS-PAGE经考马斯亮蓝复合染色,Gel-Pro蛋白系统分析,测定了γ-聚谷氨酸分子量为148ku。
Poly-γ-glutamic acid,which is metabolically produced by microorganisms,is a water-soluble,biodegradable and atoxic high molecular polyer.As a multifunctional characteristic product,poly-γ-glutamic acid has been widely used in the fields of biomedicine,food processing and cosmetics industries.In this work,γ-PGA was produced by fermentation in soybean culture and characterized by thin-layer chromatography for component,SDS-PAGE for purification,molecular weight and and UV-vis spectrum for absorption and concentration of γ-PGA.
出处
《中国酿造》
CAS
2012年第5期148-150,共3页
China Brewing
