摘要
目的 :研究辐射对伤口愈合影响的规律和机制。方法 :采用宏观、光镜、电镜及免疫组化(显示Ⅲ型胶原 )和原位杂交 (检测Ⅰ、Ⅲ型胶原mRNA)等方法 ,动态观察了大鼠放射复合伤口愈合的病理变化过程。结果与结论 :结果显示 ,伤后即刻予以 15Gy局部照射对伤口愈合有明显的延迟作用 ,具体表现为 :①伤口愈合早期炎症反应明显受到抑制 ,特别是单核细胞和中性粒细胞渗出减少 ,坏死组织增多 ,出血广泛 ;②肉芽组织生长成熟缓慢 ,成纤维细胞受到严重损害 ,出现畸形的“放射性成纤维细胞” ,胶原蛋白合成和分泌受到抑制 ;③上皮覆盖过程滞后 。
Objective: To study the regularities and mechanism of the effect of radiation on wound healing. Methods: The pathological changes in radiation combined wound healing in rats were observed by macroscopic, microscopic, electron microscopic examinations, and examination of collagen types Ⅰ and Ⅲ. Results and conclusion: It was found that wound healing process was impaired and delayed obviously in irradiated rats. Firstly, the inflammatory responses in early phase were inhibited seriously, particularly the numbers of infiltrating monocytes and neutrophils were decreased in irradiated wounds, blood vessels were injured and hemorrhage increased. Secondly, the formation and maturation of granulation tissue were slowed down, fibroblasts were damaged seriously, “radiation fibroblasts” appeared, and synthesis and secretion of type Ⅰ and type Ⅲ collagens were decreased. Lastly, reepithelialization process was delayed and healing time was prolonged. [
出处
《军事医学科学院院刊》
CSCD
北大核心
2000年第1期40-43,48,共5页
Bulletin of the Academy of Military Medical Sciences
基金
总后勤部重点招标课题资助
