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细胞培养稳定同位素标记技术的发展及其在脑病研究中的应用 被引量：3

Development of stable isotope labeling technique and its application in encephalopathy research
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摘要 寻找脑中特异的蛋白标记物,对于脑病临床诊断及药物机制研究至关重要。人脑是一个复杂的有机体,采用单靶点、非动态、局部的方法不适于脑病研究,而以质谱技术为基础的定量蛋白质组学的出现和发展,加快了脑病标志分子的研究进程。细胞培养稳定同位素标记技术(SILAC)作为精确的蛋白定量方法,是系统、动态的研究方法,为脑病机制和治疗脑病中药的研究提供了新的思路。 It is crucial to find out the brain specific protein markers for clinical diagnosis and drug mechanism.Human brain is a complex organism,so the single-target,non-dynamic and local method is not suitable for brain research.The development of quantitative proteomics,which is based on the mass spectrum,greatly accelerates the research process.As the accurate quantitative method of protein,the stable isotope labeling with amino acids in cell culture（SILAC） is a system,dynamic technology.It can provide new ideas for the mechanism research and the treatment strategy of traditional Chinese medicine in encephalopathy.
作者 朱丽 刘安 杨洪军 陈畅 李德凤 吴传鸿 高健 李韶菁
机构地区 江西中医学院 中国中医科学院中药研究所 河北大学药学院
出处 《中国药理学通报》 CAS CSCD 北大核心 2012年第7期897-900,共4页 Chinese Pharmacological Bulletin
基金 国家自然科学基金资助项目(No 30901976) 国家十二五"重大新药创制"科技重大专项资助项目(No2012ZX09103-201-055)
关键词 定量蛋白质组学 SILAC 脑病 神经血管单元 中药 网络药理学 quantitative proteomics； SILAC； encephalopathy； neurovascular unit； traditional Chinese medicine； network pharmacology
分类号 R5 [医药卫生—内科学] R322.81 [医药卫生—人体解剖和组织胚胎学]
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参考文献7

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二级参考文献24

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中国药理学通报
2012年 第7期

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