摘要
目的:观察电针预处理对再次心肌缺血家兔心肌酶的影响,探讨电针防治家兔再次心肌缺血的有效性及其可能的作用机制。方法:40只健康家兔随机分为正常组、心肌缺血组、电针预处理组、再次心肌缺血组、电针预处理心肌缺血组,每组8只。经家兔耳缘静脉注射垂体后叶素及持续滴注垂体后叶素溶液造成急性心肌缺血模型。电针预处理组在造模7d前开始每日给予"内关"穴电针预治疗,电针预处理心肌缺血组在第1次造模成功后第3天开始给予"内关"穴电针预治疗7d。记录造模后30min的心电图,生物化学法检测血清心肌酶的变化。结果:与正常组比较,心肌缺血组和再次心肌缺血组造模后30min心电图STⅡ段电位明显升高(均P<0.01);与相应的造模组比较,电针预处理均可明显降低STⅡ电位值(均P<0.01)。与正常组比较,心肌缺血组和再次心肌缺血组各项心肌酶指标均显著升高(均P<0.01);与相应的造模组比较,电针预处理可明显减少各项心肌酶的释放(均P<0.01)。电针预处理组与电针预处理心肌缺血组比较,各项指标的差异均无统计学意义(P>0.05)。结论:电针预处理通过控制心肌酶的释放对家兔再次心肌缺血损伤具有良好的保护作用。电针预处理体现了未病先防、既病防变的思想。
Objective To observe the effect of electroacupuncture （EA） preconditioning of ＂Neiguan＂ （PC 6） on myocardial enzyme levels in acute recurrent myocardial ischemia rabbits so as to study its mechanisms underlying protection of ischemic myocardium. Methods A total of 40 healthy rabbits were randomized into normal control, myocardial ischemia （MI） model, EA preconditioning （EA-P）, recurrent MI （RMI）, EA-P ＋ RMI groups. MI model was duplicated by auricular intravenous administration of Posterior Pituitary Injection（PPI, 0.3 mL/kg）. EA（2 Hz/70 Hz, 2-4 mA）was applied to bilateral PC 6 for 30 rain, once daily for 7 d before MI. The contents of serum aspartate aminotransferase（AST）, isoenzymes of creatine kinase（OK- MB）, lactate dehydrogenase（LDH）, creatine kinase（OK）, a-hydroxybutyrate dehydrogenase （^-HBD） were detected by automatic biochemistry analyzer. Results Following administration of PPI, ECG-STe, serum AST, CK-MB, LDH, OK and a-HBD levels were increased significantly in the MI and RMI groups（P〈0.01）. Compared with the MI model group, ECG-STI , serum AST, CK-MB, IDH, OK and a-HBD levels were down-regulated considerably in the EA-P group（P〈0.01 ）, and ECG-STe , serum AST, CK-MB, LDH, OK and a-HBID levels were obviously lower in the EA-P ＋ RMI group than in the RMI group（ P〈0.01 ）. No significant differences were found between the MI and RMI groups, and between EA-P and EA-P ＋ RMI groups in the abovementioned 6 indexes（ P〉0.05）. Conclusion Electroacupuncture preconditioning has a remarkable effect in the prevention of ischemic myocardial injury of both acute and recurrent myocardial ischemia in the rabbit, which is closely associated with its functions in lowering serum AST, CK-MB, LDH, CK and a-HBD contents.
出处
《针刺研究》
CAS
CSCD
北大核心
2012年第3期224-228,共5页
Acupuncture Research
关键词
电针预处理
内关穴
急性心肌缺血
心肌酶治未病
Electroacupuncture preconditioning
Neiguan （PC 6）
Acute myocardial ischemia
Myocardial enzymes
Preventive treatment
