摘要
目的:以实时荧光定量PCR(FQ-PCR)检测可疑患者HLA-B27的定量水平,研究强直性脊柱炎(AS)与HLA-B27的相关性,明确HLA-B27的检测值范围并用于AS的确诊诊断。方法:针对本院2005年～2010年收集的168例骶髂关节及下腰部疼痛等症状疑似AS病例进行回顾性分析,获得所有病例外周静脉血标本,然后通过实时荧光定量PCR进行HLA-B27的定量检测,疑似病例分为AS患者、HLA-B27阳性的非AS患者、HLA-B27阴性的非AS患者三组,根据临床体征、影像学进行确诊病情,同时选取健康体检者52例外周静脉血标本的HLA B-27测定结果进行对照,统计分析各变量与AS存在之间的关系。结果:AS患者、HLA-B27阳性的非AS患者、HLA-B27阴性的非AS患者、健康体检者的HLA-B27循环阈值(ct)平均值分别为:26.3 cop-ies/ml、17.5 copies/ml、6.2 copies/ml、4.9 copies/ml,单变量分析表明,HLA-B27与化脓性关节炎明显相关(P<0.05)。结论:AS患者HLA-B27定量分析循环阈值(ct)的95%参考值范围为(17.3～35.3)copies/ml,HLA-B27定量分析可作为疾病发病的参照影响因素及预测因素。
Objective A fluorogenic quantitative polymerase chain reaction（FQ-PCR） was used to detect quantitative level of the suspicious ankylosing spondylitis patients, to determine the relativity between ankylosing spondylitis and HLA-B27, and the predicted referenee value got from this experiment was used to diagnose ankylosing spondylitis. Methods 168 suspieous ankylosing spondylitis cases with the pain of sacroiliac joints and lower waist eolleeted from 2002 to 2010, was to do retrospective analysis, all the blood spec- imen was got to analyze the quantitative level of HLA-B27. All patients on the basis of clinie and this experiment were divided into 3 groups： AS patients, non-AS patients with HLA-B27-positive patients group, non-AS patients with HLA-B27-negative patients group. 52 cases were collected as control group. Statistieal analysis was to analyze the sensitive value and relationship with ankylosing spondy- litis. Results The ct value of HLA-B27 in AS patients, non-AS patients with HLA-B27-positive patients group, non-AS patients with HLA-B27-negative patients group were 26.3,17.5,6.2,4.9 respectively. Univariate analysis showed that HLA-B27 and septic arthritis was signifieantly related（P 〈 0.05 ）. Conclusion The quantitative level of ct-value （ HLA-B27 ） of 95% reference range in AS pa- tients was from 17.3 to 35.3. HLA-B27 quantitative analysis ean be used to diagnose and predict the patient＇s condition.
出处
《放射免疫学杂志》
CAS
2012年第3期319-322,共4页
Journal of Radioimmanology
