摘要
目的探讨Toll样受体4(Toll-like receptor4,TLR4)在不同人群外周血白细胞的差异表达及其在强直性脊柱炎(ankylo-sing spondylitis,AS)中的意义。方法应用流式细胞术检测60例AS患者及40例健康对照外周血淋巴细胞、单核细胞和中性粒细胞TLR4的表达。结果TLR4在外周血淋巴细胞、单核细胞和中性粒细胞均有表达,以单核细胞表达为主;HLA-B27阳性和阴性AS患者外周血3种细胞TLR4的阳性表达率均明显高于对照组(P<0.001),但两者之间并无显著差异。结论TLR4主要表达于外周血单核细胞,但AS患者外周血3种细胞表达均上调,这种上调与HLA-B27阳性与否无关,提示TLR4信号通路的异常与AS的发病相关。
Objective To explore the differential expression of Toll-like receptor4 （TLR4） in human leukocytes and its significance in the patients with ankylosing spondylitis （AS）. Methods Using flowcytometry we determined the expression of TLR4 on the peripheral blood leukoeytes from 60 AS patients and 40 healthy controls. Results The positive rate of TLR4 in leukoeytes of AS group was significantly higher than that in controls （ P 〈 0. 001 ）. No statistical difference was found between the HLA-B27-positive and HLA-B27-negatire AS patients （ P 〉 0.05 ）. However, both AS groups showed significantly higher positive rate of TLR4 in leukocytes than the controls （P 〈 0. 001 ）. Conclusions TLR4 is expressed in three kinds of leukoeytes （lymphoeytes, monoeytes and polymorphonuelear leukoeytes） ,but it is mainly expressed in human monoeytes. The positive rate of TLR4 on monoeytes is significantly increased,and no correlation between the HLA-B27-positive and negative patients. The results indicated that abnormal TLR4 signal pathway might be related to the pathogenesis of AS.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2007年第4期293-294,共2页
Chinese Journal of Clinical Laboratory Science
