摘要
目的:了解一起食物中毒中分离到的金黄色葡萄球菌(Staphylococcus aureus,SA)的生物学特性及其肠毒素基因检测。方法:采用VITEK-32全自动微生物鉴定/药敏分析系统对分离到的金黄色葡萄球菌菌株进行生化鉴定和药敏试验,并采用mini-VIDAS全自动荧光酶标免疫仪对菌株进行肠毒素检测,再采用PCR技术对产肠毒素的菌株进行基因分型。结果:总共分离到的19株金黄色葡萄球菌,所有分离株均对青霉素G耐药,均分泌β-内酰胺酶,肠毒素基因分型检测均为SEA与SEE。结论:19株分离株均携带肠毒素,经鉴定均为SEA与SEE肠毒素混合型,由此对食物中毒的原因有一个较圆满的解释。
Objective：To detect Staphylococcus aureus isolated from a food poisoning,then analyse biological characteristics and characterize enterotoxin genotype of the isolates.Methods： Biochemical characteristics and drug sensitive test were conducted by VITEK-32 automated microbial identification/susceptibility analysis system,then enterotoxin genes were detected by mini-VIDAS and characterized genotype by PCR.Results： 19 strains of S.aureus were isolated from samples.All S.aureus were resistant to penicillin G and can secrete β-lactamase.The enterotoxin gene types were SEA and SEE.Conclusion： The enterotoxin gene type of S.aureus isolates was mixed type of SEA and SEE.That is a better explanation about food poisoning.
出处
《中国卫生检验杂志》
北大核心
2012年第5期1063-1064,1068,共3页
Chinese Journal of Health Laboratory Technology
