摘要
目的探讨以微生物学和分子生物学角度寻找金黄色葡萄球菌引起的食源性疾病的病因。方法分别以食品卫生微生物检验国家标准(GB／T4789)分离鉴定菌株、K-B法进行药敏试验、mini-VIDAS全自动荧光酶标免疫测试仪进行葡萄球菌肠毒素检测和RiboPrinter全自动微生物基因指纹鉴定系统进行基因分型对一起由金黄色匍萄球菌引起的中毒事件进行分析。结果从来源于各类共17份样品的菌株中,均检出葡萄球菌肠毒素,对青霉素G100％耐药,对其他10种抗生素耐药率低于30％,RiboPrinter全自动微生物基因指纹鉴定系统结果证实为一起同型金黄色葡萄球菌的爆发。结论结合微生物学和分子生物学检测结果,确认这起食源性疾病是由产生肠毒素的金黄色葡萄球菌污染食物所引起。
Objective To look for the pathogen resulting in a food- borne disease by using molecular biologiy methods. Methods The suspected samples were tested to determine if they were contaminated with pathogenic bacteria according to the national standards for microbiological examination of food hygiene （GB/T4789） and drug resistance was tested by using K- B method and mini - VIDAS apparatus were utilized to determine Staphylococcal enterotoxin in the process and using RiboPrinter Automated Molecular Typing System for genotype typing. Results Staphylococcal enterotoxin was detected from the strains. The resistance of isolated strains to penicillin G was 100%, while the resistance to other 10 drugs was lower than 30% . Molecular biological results showed that it was an outbreak caused by Staphylococcus aureus. Conclusion The results of microbiological examination, together with molecularbiological show that this food - borne disease was due to contamination with enterotoxin of Staphylococcus aureus.
出处
《中国热带医学》
CAS
2006年第3期523-524,506,共3页
China Tropical Medicine
基金
广东省科技资金扶持项目
肠道致病菌基因指纹图谱库的建立和耐药性研究
项目编号:2003B0127
