摘要
应用转化转导法把含有沙门氏菌 相鞭毛蛋白基因 Flj Benx的重组质粒 p H 1 0 4导入无鞭毛的减毒株 SL592 8(Δaro,Fli Cgp∶∶ Tn1 0 )中获得表达 ;经电镜观察、玻板凝集试验及动力抑制试验证实重组菌表达了相应的外源鞭毛蛋白 ,SDS PAGE测得表达产物分子量为 52 kd;Western blot试验中表达产物可与 H 2 enx单因子血清发生特异性反应 ;体内外试验表明重组菌很稳定 ,口服免疫小鼠能诱导产生针对表达产物的特异性抗体 .
Recombinant plasmid p H 1 0 4carrying the Flj Benx gene was used to transform L B5 0 0 0 and then transferred to a flagellin-negative live vaccine strain of SL5 92 8(Δaro,Fli Cgp::Tn1 0 ) by using phage P2 2 (HT,int- ) .The flagellin gene was expressed in SL5 92 8,which was analyzed by both motility test and plate agglutination test. SDS-PAGE showed that expressed protein had molecular weight value of 5 2 kd,and western blotindicated thatthe expressed productreacted with H 2 enx factor-specific antiserum.The recombinantstrain was stable and safe.Mice immunized oral- ly with the live recombinantbacteria developed antibody specific to the expressed productas detect- ed by EL ISA.
出处
《扬州大学学报（自然科学版）》
CAS
CSCD
2000年第1期23-26,共4页
Journal of Yangzhou University：Natural Science Edition
基金
国家自然科学基金!资助项目 (396 70 5 5 7)
农业部"九五"重点项目!(95牧 0 10 30 5 )
