摘要
目的对2011年8月采集的从老挝输入的登革热病人急性期血清进行登革病毒分离,以找出病原学证据。方法用C6/36白纹伊蚊细胞进行病毒分离,对有细胞病变的标本进行RT-PCR,检测登革病毒RNA及型别,对PCR产物测序,进行比对分析和进化树分析。结果从8例输入登革热病人的血清中分离到4株能引起细胞病变的病毒,均为登革病毒1型,与泰国毒株的同源性>99%。结论云南省首次从输入登革热病人血清中分离到登革病毒1型,为云南省登革热的防控提供了依据。
Objective To confirm dengue infection and type the dengue virus in cases of dengue imported to Yunnan Province from Lao PDR in 2011.Methods Isolation of the dengue virus was done using C6/36 cells.Dengue nucleic acid was detected in the isolates via a stable cytopathic effect on C6/36 cells according to RT-PCR.The dengue virus E gene was sequenced and analyzed using BLAST.Phylogenetic analyses based on the E gene of new viral isolates were conducted using MEGA4.Results Four dengue virus isolates were obtained from eight serum samples.All were identified as dengue virus type 1.The nucleotide homology with dengue virus type 1 isolated from Thailand was greater than 90%.Conclusion This is the first isolate of dengue virus type 1 from dengue cases imported to Yunnan Province from Lao PDR.These results provide scientific evidence for dengue control in Yunnan Province.
出处
《中国病原生物学杂志》
CSCD
北大核心
2012年第4期241-243,共3页
Journal of Pathogen Biology
基金
国家自然科学基金项目(No.30960327)
关键词
登革热
登革病毒1型
输入病例
云南省
Dengue fever； dengue virus type 1； imported cases； Yunnan Province,China
