摘要
用中国药品生物制品检定所检定合格的国内外HBsAgEIA试剂及ABBOTT抗 HBs、抗 HBcEIA试剂 ,对所收集的 13份HBsAg疑难判定血清进行检测 ,并用PCR方法检测血清中HBVDNA ,结果显示国内外HBsAg试剂对部分HBVDNA阳性样品的检出率差异较大 ,提示这些样品可能为S基因突变株或HBsAg含量较低 ,因此 ,HBsAgEIA试剂的敏感度仍有待进一步提高 ,并应进一步研制检测S基因突变株的HBsAg诊断试剂。
Thirteen indeterminate HBsAg samples were tested with 21 sorts of HBsAg kits, anti HBs and anti HBc kits. HBV DNA was detected with nested PCR. The results showed that all of HBsAg kits can not easily detected HBsAg from some HBV DNA positive samples. It indicated that HBsAg concentration may be lower or HBV may be S mutants in those samples. So it is necessary to improve the sensitivity of HBsAg and to develop the diagnostic kit to detect HBV S mutants.
出处
《中国病毒学》
CSCD
2000年第1期8-10,共3页
Virologica Sinica
