摘要
通过Western-Blot发现,多克隆抗-HBs抗体及单克隆抗体B_(13),A_1均识别24kd及27kd两条多肽带,而单克隆抗体B_6,B_9及B_(12)只识别24kd,不识别27kd的多肽带。用这几株单克隆抗体对不同来源HBsAg a决定簇的表位密度进行了研究,结果表明血源、重组CHO细胞表达及重组痘苗病毒表达的HBsAg与重组酵母表达的HBsAg在结合单克隆抗体A_1、B_6及B_9的密度上有数量的差别,并且有统计学意义。
Five selected McAbs were tested for their binding to HBsAg polypeptidecomposition by using Western Blot. McAb B_6, B_9 and B_(12) combined polypeptideP24 of HBsAg, while McAb A_1 and B_(13) combined both polypeptide p24 andP27 of HBsAg. Using the five McAbs, the epitope density of 'a' determinantof HBsAg derived from different sources was also studied with the second an-tibody method. The result showed that there was similar density of epitopesagainst five McAbs on plasma-derived HBsAg (PD HBsAg), CHO cell-derivedHBsAg (CD HBsAg ) and vaccinia-derived HBsAg (VD HBsAg). There was alsosimilar density of epitopes on two preparations of yeast-derived HBsAg (YDHBsAg) produced by Amgen Co. and Phillipe Co. respectively. However epi-topes against three McAbs (B_6, B_9 And A_1) on YD HBsAg were quantitativelydifferent from those on PD HBsAg, CD HBsAg and VD HBsAg. This analysisof HBsAg epitopes may be important for the evaluation of their immunogen i-sity.
出处
《中国病毒学》
CSCD
1992年第1期48-53,共6页
Virologica Sinica
