摘要
哺乳动物生精过程是一个精细的调节过程 ,人们对于这个过程还了解甚少。这主要是由于维持生殖细胞体外培养的必需条件尚未建立 ,睾丸生精细胞可原代培养的时间太短。因此 ,有必要建立能在体外长期传代的睾丸生精细胞株。Hofmann等人用磷酸钙法将猴病毒 40大T抗原 (sv40lt)基因掺入小鼠睾丸细胞建立了永生的睾丸细胞株 ,并利用这些细胞株研究了睾丸特异基因的表达。随后 ,又通过对小鼠睾丸生精细胞共转染sv40lt基因和编码温度敏感p5 3蛋白基因 ,建立了两个能在体外继续分化的生精细胞株。这些睾丸细胞株的建立为精子发生研究提供了一个有用的实验模型。
Spermatogenesis is a precisely regulated process in the mammalian testis,but little is known about it.This is mainly because the in vitro conditions necessary for the maintenance of germ cell development have not been established and the duration of primary cultures of testicular cells is too short.Therefore,the establishment of testicular germ cell lines able to proliferate in vitro over long period of time would be necessary.Hofmann and his colleagues have introduced simian virus 40 large T antigen ( sv40 lt )gene into mouse testicular cells by Ca 3(PO 4) 2 method and established immortalized cell lines.Using these cell lines,they have studied the expression of some testis specific genes.Later,two more germ cell lines able to differentiation in vitro have been established by cotransfecting primary mouse testicular germ cells with sv40 lt gene and the gene coding for a temperature sensitive mutant of p53.The establishment of these testicular cell lines offers a powerful experimental model and will promote our understanding of the mechanism of spermatogenesis.
出处
《中华男科学杂志》
CAS
CSCD
2000年第1期43-46,共4页
National Journal of Andrology
