摘要
运用开顶式气室(OTCs)模拟当前环境大气O3浓度(对照,40～45 nL.L-1)、O3浓度倍增1倍(TR-1,92～106 nL.L-1)和O3浓度倍增2倍(TR-2,142～160 nL.L-1)胁迫,以叶片光合色素、MDA、可溶性蛋白质和可溶性糖含量及SOD和POD活性和相对电导率为指标,分析了美丽箬竹(Indocalamus decorus Q.H.Dai)对O3胁迫的生理响应规律。结果显示:随O3浓度的提高,叶片叶绿素a(Chla)、叶绿素b(Chlb)、总叶绿素和类胡萝卜素含量,SOD和POD活性,可溶性蛋白质和可溶性糖含量及二者的比值(SPC/SSC)均呈下降趋势;叶绿素a与b的比值(Chla/Chlb)、MDA含量和相对电导率均呈增加趋势。TR-1条件下叶片POD活性和可溶性糖含量分别比对照下降38.86%和10.37%,差异显著;Chla/Chlb比值显著高于对照,其余指标均与对照无显著差异。而在TR-2条件下叶片Chla、Chlb、总叶绿素和类胡萝卜素含量显著低于对照,降幅分别为26.89%、40.91%、30.47%和20.37%;SOD和POD活性、可溶性蛋白质和可溶性糖含量以及PC/SSC比值也均显著低于对照,降幅分别为42.03%、46.62%、45.09%、11.52%和40.15%;Chla/Chlb比值、MDA含量和相对电导率均显著高于对照。另外,TR-1与TR-2处理组间光合色素含量和POD活性差异不显著,TR-2处理组MDA含量和相对电导率显著高于TR-1处理组,SOD活性显著低于后者。研究结果表明:美丽箬竹对O3胁迫具有强耐受性,可种植于O3浓度较高的环境中。
The method of open-top chambers （OTCs） was used to simulate ozone stress with atmospheric 03 concentration （control, 40-45 nL L-X）, one time 03 concentration （TR-1, 92-106 nL .L-l） and two times 03 concentration （TR-2, 142-160 nL . L-1）, and taking contents of photosynthetic pigment, MDA, soluble protein and soluble sugar, activities of SOD and POD and relative electric conductivity as indexes, physiological response of Indocalamus decorus Q. H. Dai to 03 stress was researched. The results show that with rising of O3 concentration, contents of chlorophylla （ Chla）, chlorophyllb （ Chlb）, total chlorophyll and carotenoid, activities of SOD and POD, soluble protein content （SPC） and soluble sugar content （SSC） and SPC/SSC value in leaves of L decorus appear the reducing trend, while Chla/ Chlb value, MDA content and relative electric conductivity appear the enhancing trend. In TR-1 group, POD activity and SSC decrease respectively 38.86% and 10.37% comparing to the control with the significant difference, Chla/Chlb value is significantly higher than that of the control, but the other indexes have no significant difference with the control. In TR-2 group, contents of Chla, Chlb, total chlorophyll and carotenoid are significantly lower than those of the control with decreasing ranges of 26.89% , 40.91%, 30.47% and 20.37%, respectively, activities of SOD and POD, SPC and SSC andSPC/SSC value also are significantly lower than those of the control with decreasing ranges of 42.03%, 46.62%, 45.09%, 11.52% and 40. 15%, respectively, but Chla/Chlb value, MDA content and relative electric conductivity are significantly higher than those of the control. Moreover, photosynthetic pigment content and POD activity have no significant differences between TR-1 and TR-2 groups, but MDA content and relative electric conductivity in TR-2 group are significantly higher and SOD activity in TR-2 group is significantly lower than those in TR-1 group. It is revealed that L decorus possesses a strong resistance to 03 stress, and can be cultivated in high 03 concentration environment.
出处
《植物资源与环境学报》
CAS
CSCD
北大核心
2012年第2期68-72,88,共6页
Journal of Plant Resources and Environment
基金
中央级公益性科研院所基本科研业务费专项资金项目(RISF6915)
国家林业公益性行业科研专项(201004008)
浙江省省院合作资助项目(2010SY01)
关键词
美丽箬竹
臭氧胁迫
生理响应
光合色素
抗氧化酶
渗透调节物质
Indocalamus decorus Q. H. Dai
ozone stress
physiological response
photosyntheticpigment
anti-oxidation enzyme
osmotic adjusting substance
