摘要
目的 探讨食管鳞癌细胞凋亡和增殖细胞核抗原的表达及其临床意义。方法 采用原位DNA末端标记法和免疫组化法分别检测 70例食管鳞癌的细胞凋亡和增殖细胞核抗原的表达情况 ,计算出凋亡指数和增殖指数。结果 凋亡指数及其与增殖指数的比值与肿瘤的病理分级和淋巴结转移密切相关 (P <0 .0 5 )。结论 食管癌的发生发展与细胞凋亡和增殖失控有关。
Objective To study the relationship between apoptosis and PCNA expression in esophgeal squamous cell carcinoma and clinicopathological characteristies of the tumor.Methods Apoptosis and PCNA expression were detected with TdT mediated dUTP biotin nick end Labeling(TUNEL) and immunohistochemistry method.Apoptosis and PCNA indexs were respectively the percentages of apoptotic cells or PCNA positive cells among tumor cells.Results Apoptosis indexs and the ratio of apoptosis indexs to PCNA indexs were well correlated with the pathlogical grading and lymph node metastasis(P<0.05).Conclusion The abnormal change of cell apoptosis and proliferation play an important role in the development of esophageal carcinoma.
出处
《实用癌症杂志》
2000年第3期264-266,共3页
The Practical Journal of Cancer
关键词
食管肿瘤
细胞凋亡
增殖细胞核抗原
Esophageal neoplasms
Apoptosis
Proliferating cell nuclear antigen(PCNA)
