摘要
目的研究表浅膀胱移行细胞癌中细胞凋亡及增殖细胞核抗原（ＰＣＮＡ）表达情况，并初步探讨其与表浅膀胱移行细胞癌复发的关系。方法采用原位ＤＮＡ末端标记法及免疫组织化学方法检测了８７例表浅膀胱移行细胞癌中细胞凋亡及ＰＣＮＡ表达情况，计算出细胞凋亡指数及ＰＣＮＡ表达指数，并对复发组与非复发组进行比较。结果复发组与非复发组细胞凋亡指数差异无显著意义，而凋亡指数与ＰＣＮＡ指数比值在复发组小于非复发组（Ｐ＜００１），显示在复发组癌细胞的增殖程度明显高于细胞凋亡程度。结论对凋亡指数及ＰＣＮＡ阳性指数的综合分析，即凋亡指数与ＰＣＮＡ阳性指数的比值，可更全面的反映细胞凋亡与增殖的平衡状态，对判断肿瘤的复发具有参考价值。
Objective To determine the expression of apoptosis and PCNA in superficial transitional cell bladder cancer and to evaluate its significance as a recurrence marker. Method 87 cases of superficial transitional cell bladder cancer were analysed for the expression of apoptosis and PCNA by using the 3end labeling method of DNA and immunohistochemical staining in tissue sections. The apoptotic indexes and PCNA indexes were respectively the percentages of apoptotic cells or PCNA positive cells among tumor cells. Result The end labeling method allowed a precise evaluation of the expression of apoptosis. Apoptosis occurred in 896% of superficial transitional cell bladder cancer. In 17 patients who suffered from tumor recurrence,the ratio of apoptosis indexes to PCNA indexes was significantly higher than that in those without recurrence (P<001). Conclusion The ratio of apoptosis indexes to PCNA indexes was believed to be related to tumor recurrence and might be of value in the assessment of recurrence of superficial transitional cell bladder cancer.
出处
《中华外科杂志》
CAS
CSCD
北大核心
1998年第8期484-486,共3页
Chinese Journal of Surgery
基金
广东省重点科技攻关项目基金
关键词
膀胱肿瘤
PCNA
移行上皮癌
细胞凋亡
Bladder neoplasms Proliferating cell nuclear antigen Apoptosis
