摘要
目的 探讨Fas、Bax蛋白在胃腺癌中的表达及其与细胞凋亡的关系。方法 采用免疫组织化学ABC法及细胞凋亡TUNEL法检测 48例胃腺癌中Fas、Bax蛋白的表达及细胞凋亡。结果 Fas ,Bax蛋白在高、中分化胃癌中的阳性表达率显著高于低分化胃癌 (P <0 .0 5 ) ;Fas、Bax蛋白在有淋巴结转移癌中的阳性表达率均为 2 3 .8% ,显著低于无淋巴结转移癌的 5 5 .8%和5 1.9% (P <0 .0 5 ) ,Fas蛋白在侵及粘膜及粘膜下层胃癌中的阳性表达率高于侵及浆膜及周围组织的胃癌 (P <0 .0 5 ) ,而Bax蛋白与浸润深度无明显关系 ;Fas蛋白与Bax蛋白在胃癌中的表达呈显著相关性 (P <0 .0 5 )。Fas、Bax蛋白阳性组织与Fas、Bax蛋白阴性组织比较 ,前者凋亡指数明显高于后者 (P <0 .0 5 )。结论 Fas、Bax异常表达与胃癌的分化、预后有关 ,两者在胃癌中的表达呈相关性 ,并且对细胞凋亡均有重要调节作用。
Objective To study the role of Fas、Bax and apoptosis in gastric cancer.Methods The expression of Fas、Bax and apoptosis was examined with avidin biotin complex(ABC) and TUNEL Technique in 48 specimens of gastric cancer.Results The positive rates of Fas and Bax were higher in mediated differentiated and well differentiated groups than in poorly differentiated group( P <0.05),it was lower in Iymphnode metastasis(23.8%) than in non metastasis(55.8%,51.9% respectively, P <0.05).The positive rate of Fas in invasion of mucosa and submucosa(71.4%) was higher than that in invasion of serosa and surrounding tissues(23.5%, P <0.05);The expression of Bax was not related to the depth of invasion.In gastric carcinoma,significant correlation existed between Fas and Bax expression because Fas positive reaction was observed in 20/48 cases assayed,of which 17 exhibited Bax positive.Apoptotic index was higher in Fas、Bax postive tissues than in negative ones( P <0.05).Conclusion There may be a positive correlation between the expression of Fas、Bax and differentiation,prognosis of gastric cancer.The Fas and Bax may be an important regulator to cell apoptosis.
出处
《实用癌症杂志》
2000年第3期238-240,共3页
The Practical Journal of Cancer
基金
河南省科委科研基金!(No .981 1 70 2 0 3)
关键词
FAS蛋白
BAX蛋白
胃癌
浸润转移
细胞凋亡
Fas protein
Bax protein
Gastric cancer
Infiltration and metastasis
Apoptosis
