摘要
采用强优势玉米杂交种苏玉16(JB×Y53)的两个亲本自交系,配置F2和相应F2∶3作图群体。利用154个SSR标记构建了分子标记连锁图谱,覆盖全基因组1 735.0 cM,标记间平均图距为11.3 cM。同时考察F2和F2∶3群体的株高、穗位高、抽穗期和散粉期等共10个重要农艺性状,采用联合F2和F2∶3群体的作图方法定位有关QTL。此外,采用QTL Cartographer V2.5软件分别对F2和F2∶3群体进行了有关QTL的重演性验证。结果表明,采用联合作图方法在调查的10个性状上共定位到93个QTL,采用QTL Cartographer V2.5软件共定位到96个QTL,其中56个能用两种方法重演验证。
The maize inbred lines JB and Y53,the parental lines of maize hybrid Suyu 16,were used to create F2 and F2∶3 segregation populations.The genetic linkage map was constructed containing 154 SSR markers,which covered 1 735.0 cM of maize genome.The average distance of the flanking markers was 11.3 cM.The phenotypic value of 10 traits including plant height,ear height,heading date,pollen shedding,etc were investigated in F2 and F2∶3 populations.The QTL associated with these traits were mapped through jointing F2 and F2∶3 populations.The composite interval mapping（CIM） in software QTL Cartographer V2.5 was also used to map the QTL in F2 and F2∶3 populations,respectively.93 QTLs were identified in the 10 traits by joint mapping method,and 96 QTLs were identified by CIM method.56 QTLs among 93 QTLs could be verified by CIM method.
出处
《江苏农业学报》
CSCD
北大核心
2012年第2期265-273,共9页
Jiangsu Journal of Agricultural Sciences
基金
国家重大基础研究发展规划(973计划)项目(2011CB100106)
国家自然科学基金项目(30971846、31100882)
江苏省高校自然科学基金重点项目(09KJA210002)
