摘要
In the current study,caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens.Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction,followed by purification using a macroporous resin (D101 type) column and silica gel chromatography.The faint-yellow caffeic acid product was yielded with a purity of 98.46%,and it was chemically identified from spectra of Fourier transform infrared spectroscopy (FTIR),proton nuclear magnetic resonance (1 H NMR)/carbon nuclear magnetic resonance (13 C NMR),and electrospray ionization mass spectrometry (ESI-MS).Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.
In the current study,caffeic acid was an important metabolite in the highly copper-tolerant plant Elsholtzia splendens.Preparation and purification of caffeic acid were performed on the dried biomass of the plants by means of sonication/ethanol extraction,followed by purification using a macroporous resin （D101 type） column and silica gel chromatography.The faint-yellow caffeic acid product was yielded with a purity of 98.46%,and it was chemically identified from spectra of Fourier transform infrared spectroscopy （FTIR）,proton nuclear magnetic resonance （1 H NMR）/carbon nuclear magnetic resonance （13 C NMR）,and electrospray ionization mass spectrometry （ESI-MS）.Caffeic acid is a possible product from the post-harvest processing of Elsholtzia splendens biomass.
基金
Project supported by the Zhejiang Provincial Qianjiang Talents for Science and Technology (No.2011R10026)
the Education Department of Zhejiang Province (No.Y201016563)
the Research Funds from State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering (No.2009490711)
the Fundamental Research Funds for the Central Universities,China
