摘要
从土样中筛选到一株产β－聚精酶的曲霉ＦＳ６８野生菌株，经ＵＶ，ＮＴＧ等五代诱变，获得变株ＦＳＵＮ－５１。对该菌株产酶条件优化实验结果表明：最佳培养基配方（％）：大麦粉５，黄豆饼粉 ２， ＦｅＳＯ_４· ７Ｈ_２Ｏ ０． ０１， Ｎａ_２ＨＰＯ_４ ０． １， ＣａＣＯ_３ ０． ５， ＭｇＳＯ_４ ０． ０３， ＮａＮＯ_３ ０． ４；最适的发酵条件为产酶ｐＨ为６．５，温度为３１℃，在２５０ｍｌ三角瓶中装量为２５ｍｌ，发酵周期为７２ｈ，摇瓶相对酶活比野生菌株提高６９％。
FSUN-51,a high product strain of β-glucanase, was obtained through mutation breeding by UV,NTG etc' from the wild strain FS68 screened from the soil sample. Fermentation conditions such as carbon sources nitrogen sources, cultural temperature and so on were studied. The optimum conditions were as follows barley meal as carbon source, soybean meal as nitrogen source,initial pH 6. 5 and fermentation time 72 hours. The comparative enzyme activity form FSUN-51 increase 69 %.
出处
《福建轻纺》
2000年第4期1-5,共5页
The Light & Textile Industries of Fujian
基金
福建省科委资助项目
