摘要
[目的]为有效防治黄瓜枯萎病,进行了菌种鉴定及其抗菌物质性质的试验。[方法]通过形态特征、生理生化特性以及16S rDNA序列的进化树分析,对拮抗内生细菌BPT-18进行鉴定;采用平板对峙法测定抑菌谱;采用平板扩散法测定经60%硫酸铵沉淀所得粗提液的性质。[结果]鉴定菌株BPT-18为短小芽孢杆菌,该菌对多种植物病原真菌均有拮抗作用。其抗菌物质粗提液耐高温,对酸碱稳定,对胰蛋白酶、胃蛋白酶和蛋白酶K均不敏感,对氯仿敏感,并能有效抑制枯萎病菌孢子萌发。[结论]该抗菌物质可能是一种抗菌多肽,为黄瓜枯萎病的防治提供新的生防资源。
[Aims] The test was carded out to identify strains and determine the nature of an antifungal substance for an effective prevention and control of cucumber Fusarium Wilt. [Methods] Antagonistic endophytic bacterium BPT-18 were identified by analyzing morphologic characteristics, physiological and biochemical properties and partial sequence of the 16S rDNA. The antifungal spectrum was determined by activity plate assay and the nature of crude extract obtained by 60% ammonium sulfate precipitation was determined through diffusion plate assay. [Results] BPT-18 was identified as Bacillus pumilus showing activity to inhibit some pathogenic fungi. The antifi.mgal crude substance extract had high stability against heat, acid and alkali. It was not sensitive to trypsin, pepsin and proteinase K but to chloroform, and could greatly affect spore germination. [Conclusions] The antifungal substance could be a kind of antifungal peptides, which provides new biological recourse for the prevention and control of cucumber Fusarium Wilt.
出处
《农药》
CAS
北大核心
2012年第6期464-467,共4页
Agrochemicals
基金
国家农业科技成果转化基金项目(2010GB2A200041)
河北省自然科学基金项目(C2009001280)
关键词
黄瓜枯萎病
短小芽孢杆菌
鉴定
抗菌物质
cucumber Fusatium Wilt
Bacillus pumilus
identification
antifungal substance
