摘要
目的:研究比较维泰醇和紫杉醇对宫颈癌U14细胞抗肿瘤的作用,及维泰醇抗宫颈癌作用的特点及机制。方法:采用MTT法、AO/EB染色法及免疫细胞化学法等观察、比较维泰醇及紫杉醇抑制宫颈癌细胞增殖及诱导凋亡的能力。结果:二者均能显著抑制U14细胞的增殖,维泰醇抑制U14细胞增殖的能力低于紫杉醇。维泰醇、紫杉醇对U14细胞48h的IC50值分别为7.02μmol.L-1、0.71μmol.L-1。7μmol.L-1的维泰醇与0.7μmol.L-1的紫杉醇诱导宫颈癌U14细胞凋亡的能力相当。二者下调宫颈癌U14细胞内Bcl-2表达的能力相当,但7μmol.L-1维泰醇下调Bax表达的能力稍弱于0.7μmol.L-1紫杉醇。结论:维泰醇及紫杉醇均可抑制宫颈癌U14细胞增殖并诱导其凋亡,而维泰醇因其较低的细胞毒性及成本低廉有望成为新的抗肿瘤药物。
Objective：To evaluate the effect on apoptosis inducing and the proliferation inhibiting of mouse cervical carcinoma U14 cells of Alternol and Paclitaxel,and their anti-cancer effect and explore anti-cancer mechanism of Alternol.Methods： MTT assay,the acridine orange/ethidium bromide（AO/EB） double staining and immunocytochemistry were used to observe the anti-cancer ability.Results： Alternol and Paclitaxel can inhibit the proliferation of U14 cells,but Paclitaxel was more effectively.7μmol·L-1 Alternol had the same ability of inducing apoptosis on U14 cells as so as 0.7μmol·L-1 Paclitaxel,while both of them havd equal capacity of down-regulating expression of Bcl-2 proteins,but 0.7μmol·L-1 Paclitaxel had an advantage on up-regulating expression of Bax protein.Conclusion： Both Alternol and Paclitaxel can inhibit the proliferation and induce apoptosis of cervical carcinoma U14 cells effectively.Although the anti-tumor ability of Alternol is weaker than Paclitaxel,it will be expected to become a novel,effective and low cytotoxic drug in the treatment of cancer.
出处
《现代肿瘤医学》
CAS
2012年第6期1145-1149,共5页
Journal of Modern Oncology
基金
科技部重大新药创制科技重大专项(编号:2009ZX 09103-141)
关键词
维泰醇
紫杉醇
U细胞
抗肿瘤
Altemol
Paclitaxel
U14 cells
anti- tumor
