摘要
目的:观察轴突生长抑制因子Nogo-A及其受体NgR在视网膜缺血再灌注(retinal ischemia-reperfusion,RIR)急性损伤中的表达,研究两者在RIR损伤中的作用及相关性。方法:SD大鼠90只随机分为:正常对照组(n=6);假手术组(n=42);RIR组(n=42),假手术组及RIR组分为再灌注后0,6,12,24,48,72,168h亚组,每组6只;采用结扎单侧颈总动脉的方法制备大鼠RIR模型,HE染色观察组织形态学改变,免疫组织化学检测Nogo-A和NgR蛋白的表达。结果:假手术组各时间点Nogo-A及NgR表达与正常组相比无统计学差异(P>0.05),实验组大鼠与假手术组相比:Nogo-A的表达在12h开始升高(P<0.05),48h达到高峰(P<0.01),72h下降(P<0.05),168h达到正常基线水平(P>0.05);NgR的表达在6h即出现上升,持续至48h达到最高峰(P<0.01),72h下降,168h达到正常基线水平。实验组大鼠Nogo-A与NgR的表达呈正相关(P<0.01)。结论:Nogo-A和NgR在RIR各时间点均有表达,其表达水平沿时间点呈抛物线形,在再灌注48h时均达到峰值,NgR先于Nogo-A表达,两者协同作用,与RIR损伤后抑制节细胞轴突修复再生有关。
AIM：To observe the expression of Nogo-A and NgR in rat retinal ischemia-reperfusion（RIR） acute injury and to investigate their significance and correlation. METHODS： Ninety adult SD rats were randomly divided into normal group（6 rats）, experimental group（RIR group,42 rats） and sham group（42 rats）.The experimental group and sham group were subdivided into 0 hour,6,12,24,48,72 and 168 hours subgroups according to different time after RIR injury.Six rats were bring into every subgroup.Ligating the rats＇ single common carotid artery was used to establish animal models of rat retinal ischemia. Pathomorphology was detected by hematoxylin and eosin stain and the expression of Nogo-A and NgR was measured by immunohistochemistry. RESULTS： Compared with normal group, the expression of Nogo-A and NgR in sham group had no statistically significant difference （P0.05）. Compared with sham group ,experimental group had the expression of Nogo-A gradually increased in 12 hours after RIR（P0.05）, peaked in 48 hours（P0.01）,decreased in 72 hours（P0.05） and reached in normal leavel in 168 hours（P0.05）; the expression of NgR increased in 6 hours after RIR（P0.05）, peaked in 48 hours（P0.01）,decreased in 72 hours（P0.05） and reached in normal leavel in 168 hours（P0.05）. The expression of Nogo-A and NgR in experimental group showed a positive correlation（P0.01）. CONCLUSION： Nogo-A and NgR expressed at each time point after RIR as a parabola.NgR expressed prior to Nogo-A.They were all peaked at 48 hours after RIR.The expression of Nogo-A and NgR closely related to RIR injury, with a collaborative relationship between the two.
出处
《国际眼科杂志》
CAS
2012年第6期1044-1047,共4页
International Eye Science
