摘要
我们用牙龈拟杆菌参考菌株(47A-1)制备标准免疫血清,通过问接免疫荧光法直接检测人龈下菌斑的涂片,共检查了90例正常人、75例牙龈炎患者、70例成年牙周炎患者。从记录一个荧光显微镜视野中的牙龈拟杆菌菌数发现:健康人与患者的标本间有一个明显的分界线,它具有帮助临床工作者诊断的实际意义。
In this study rabbit antisera were prepared with the cells of a type strain of B.gingivalis 47A-1, samples of subgingival plaque on slides were examined with indirect immunofluorescence method from 90 periodotolly normal subjects, 75 cases with gingivitis and 70 cases with periodontitis. Under fluorescent microscope counts of fluorescent cell bodies of B.gingivalis in a microscopic field were: normal subjects (1.26±0.74), gingivitis patients(5.64±2.86), adult cases of periodontitis(77. 96±34. 76). These results showed that among three groups there existed a clear separating line.It provide a valuable information for the periotontal clinical practitioners in confirming their diagnosis. We propose that indirect immunofluorescence technique, if properly applied, is a simple and rapid, a metnod in detecting B gingivitis and a reliable means to accelerate lab. diagnosis in subgingival infections.
出处
《中国微生态学杂志》
CAS
CSCD
1990年第2期31-34,共4页
Chinese Journal of Microecology
关键词
牙龈拟杆菌
间接免疫荧光法
Bacteroides
gingivalis Indirect immunofluorescence
