摘要
对杏鲍菇(Pleurotus eryngi)i、灵芝(Ganoderma lucidum)、云芝(Coriolus versicolo)r和鸡腿菇(Coprinus coma-tus)4种食用菌进行一级液体富硒培养,采用原子荧光光谱仪(AFS)对富硒菌丝体进行总硒含量的测定,液相色谱-原子荧光光谱仪联用(LC-UV-AFS)对富硒菌丝体中硒形态进行分析,结果显示,在一级液体培养中添加50mg.L-1Na2SeO3条件下,杏鲍菇无论生物量、总硒含量还是硒利用率都是最高的,分别达到了9.8g.L-1、5 086μg.L-1和22.3%,灵芝次之,分别是6.7g.L-1、4 426μg.L-1、19.4%。硒形态分析结果揭示,在杏鲍菇菌丝体蛋白酶提取液中硒代蛋氨酸、硒代胱氨酸和硒甲基硒代半胱氨酸3种硒代氨基酸总量比较低,不足提取液总硒30%,而其他三类食用菌3种硒代氨基酸总量达到80%以上,且在4种食用菌菌丝体提取液中,硒代蛋氨酸是硒与氨基酸的主要结合形式。研究及相关文献表明灵芝是食用菌中最具潜力的富硒材料。
Pleurotus eryngii, Ganoderma lucidum, Coriolus versicolor and Coprinus comatus were cultured in selenium （Se） -enriched medium with 50mg/L Na2SeO3. Hydride Generation Atomic Fluorescence Spectrometry （HG-AFS） was employed to determine the total Se contents on harvested mycelium, and Selenocystine （SeCys2） , selenomethylseleno- cysteine （SeMeCys） , selenomethionine （SeMet） and Se （IV） species were also determined by coupled technique liq- uid chromatography-UV irradiation-hydride generation-atomic fluorescence spectrometry （LC-UV-HG-AFS） . The re- sults displayed that Pleurotus eryngii had the highest biomass, total Se concentration and Se utilization efficiency with 9.8g.L-1, 5 086μg.L-1 and 22.3%, respectively, and then Ganoderma lucidum with 6.68g.L-1, 4 426μg.L-1and 19.4%, re- spectively. However, Pleurotus eryngii had the lowest proportion of SeCys2, SeMeCys and SeMet with less than 30% of total Se content. In contrast, the proportions of SeCys2, SeMeCys and SeMet were more than 80% of total Se content in Ganoderma lucidum, Coriolus versicolor and Coprinus comatus. Moreover, SeMet was dominated in the Se species. The present study and relevant literature revealed that Ganoderm~ lucidum was the best edible fungus species for Se-bioforti- fication.
出处
《中国食用菌》
2012年第3期38-41,共4页
Edible Fungi of China
基金
国家自然科学基金(40601088)
中国博士后自然科学基金(20100480928)
关键词
食用菌
灵芝
硒
硒形态
硒代氨基酸
Edible fungus
Ganoderma lucidum
Selenium （Se）
Se species
Se amino acids
