摘要
目的 为临床提供一种快速诊断呼吸道感染病原体的方法。方法 对1318例呼吸道感染患者的血清标本应用九项呼吸道联检试剂（间接免疫荧光法）同时检测九项主要病原体的IgM抗体，包括嗜肺军团菌（LP）、肺炎支原体（MP）、Q热立克次体（COX）、肺炎衣原体（CP）、腺病毒（ADV）、呼吸道合胞病毒（RSV）、甲型流感病毒（IFA）、乙型流感病毒（IFB）和副流感病毒1、2和3型（PIVS）。结果 非典型病原体感染率为33.3％（439／1318），其中以肺炎支原体最为多见，其次为呼吸道合胞病毒，流感病毒B、A次之；混合感染率达15．7％（207／1318）；感染人群以儿童多见。结论 九项呼吸道联检试剂（间接免疫荧光法）检测快速、操作简便、准确性高，检测范围广、利于早期发现，费用不高，适合各大医院推广应用。
Objective To introduce a rapid approach for the diagnosis of pathogens in various respiratory tract infection. Methods The sera were collected from 1318 patients with respiratory tract infection and were subjected to simultaneous detection of immunoglobin bf of nine major pathogens, including Legionella pneumophila （ LP ） , Mycoplasma pneumonia （MP）, Coxiella burneti （COX）, Chlamydia p neumoniae（CP）, adenovirus（ADV）, respiratory syncytial virus（RSV）, influenza A virus（IFA）, influenza B virus B （IFB） and parainfluenza virus （PIVS） type 1, 2 and 3, via indirect immunofluorescence assay. Results Infection of atypical pathogen , predominantly MP, followed by RSV, IFB and IFA, accounted for 33.3% （439/1318）. Combined pathogen infection, to which children were predisposed, amounted to 15.7% （207/1318）. Conclusions Indirect immunofluorescence assay using nine-item combined reagent can be applied extensively as a result of rapid detection, simplified processing, high accuracy and low cost. This may be helpful upon diagnosis in a timely manner, allowing for further popularization among various hospitals.
出处
《中华生物医学工程杂志》
CAS
2012年第2期124-127,共4页
Chinese Journal of Biomedical Engineering
关键词
呼吸道感染
无特异性病原体有机物
荧光抗体技术
间接
抗体
Respiratory tract infection
Specific pathogen- free organisms
Fluorescentantibody technique, indirect
Antibodies
