摘要
目的对2004年发生在湖北省、江西省、江苏省和北京市的腺病毒暴发及散发的疫情进行流行病学总结和分析以及病原学研究,以了解中国腺病毒感染状况。方法对各地分离的共16株腺病毒毒株进行了病原学研究,包括病毒的培养,提取病毒核酸,针对Hexon蛋白基因的PCR扩增以及PCR产物的序列测定和分析,并根据流行病学资料对腺病毒的疫情特点进行总结和分析。结果2004年引起湖北省、江西省、江苏省和北京市的腺病毒暴发及散发的疫情均为腺病毒3型;分离到的16株腺病毒毒株,在所选的900bpHexon基因片段,基因变异较小,核苷酸和氨基酸的同源性在98.5%￣100%。结论目前我国学龄儿童以及低年龄组儿童的腺病毒感染主要为3型所致,各地分离的病毒株未观察到明显的基因变异。
Objective This study was designed to research the pathogen and analysis the outbreak and sporadic epidemic of adenovirus occurred in Hubei,Jiangxi,Jiangsu Province and Beijing Municipality in order to learn the infection status of adenovirus in China. Methods Pathogenic studies of 16 strains of adenovirus isolated were implemented, in which virus isolation, RNA extraction, PCR and sequencing were made. On the basis of epidemiological data, the epidemic charicteristics of adenovirus was summarized and analyized. Results The results showed that all of the 16 isolates were adenovirus 3 and no significance difference was observed among those strains. Conclusion Adenovirus type 3 is the main type that caused school ages and young children adenovirus infection and there was no significant gene mutation among these different provincial isolates.
出处
《疾病监测》
CAS
2006年第6期285-287,共3页
Disease Surveillance
基金
未知病毒的高通量快速筛查和鉴定技术研究(2003BA712A01)
关键词
腺病毒
暴发
病原学
adenovirus
outbreak
pathology
