摘要
目的探讨山茱萸多糖对人肺癌A549细胞凋亡的作用及凋亡相关基因表达的影响。方法采用MTT法、DNA琼脂糖凝胶电泳法、免疫组化SP法检测山茱萸多糖对A549细胞凋亡作用及Bcl-2、Bax蛋白表达的变化。结果山茱萸多糖对A549细胞抑制作用明显,抑制程度与质量浓度呈正相关;DNA琼脂糖凝胶电泳出现相差约200 bp的DNA梯形条带,免疫组化SP法染色显示Bcl-2的表达下降,Bax的表达上升。结论山茱萸多糖对人肺癌A549细胞的增殖有较强抑制作用,能诱导细胞凋亡,其机制与下调Bcl-2的表达,上调Bax的表达有关。
AIM To explore the effect of Corni Fructus polysaccharides on apoptosis and expression of related gene in lung carcinoma cells A549.METHODS MTT assay was used to determine A549 cells proliferation,and agarose gel electrophoresis for DNA ladder was used to detect apoptosis.And immunohistochemistry SP was used to detect the expression of Bcl-2 and Bax.RESULTS Corni Fructus polysaccharides displayed strong inhibiting effect against the cell multiplication.There were positive correlations between the inhibitory degree and concentration.DNA agarose gel electrophoresis showed about 200 bp DNA ladder-shaped strap.Immunohistochemistry SP demonstrated that Bcl-2 expression down regulated and Bax expression up regulated.CONCLUSION Corni Fructus polysaccharides have the stronger inhibiting effect on cell multiplication of A549,and can induce the cell apoptosis.The mechanism may be associated with the down-regulation of Bcl-2 expression and up-regulation of Bax expression.
出处
《中成药》
CAS
CSCD
北大核心
2012年第5期808-811,共4页
Chinese Traditional Patent Medicine
基金
保定市科技局科学技术研究与发展指导计划项目(09ZF011
09ZF004)
河北省人口和计划生育委员会科研计划项目(2009-B18
2010-A06)
河北省中医药管理局科研计划项目(2009061)
