摘要
目的 研究安氟醚和异氟醚预处理对肝脏缺血 再灌注损害的影响。方法 18只家兔随机分 3组 ,对照组阻断肝动脉和门静脉血流形成肝缺血 45min ,开放再灌注 12 0min ;安氟醚和异氟醚预处理组 ,于缺血再灌注前分别吸入 1 6 8%安氟醚和 1 15 %异氟醚 2 0min和药物清除 10min。结果 缺血前 ,三组血清谷丙转氨酶 (ALT)、谷草转氨酶 (AST)、乳酸脱氢酶 (LDH)、碱性磷酸酶 (ALP)和谷氨酰酶 (GGT)活性无统计学差异 ;再灌注 10min后 ,除安氟醚组预处理AST升高程度明显低于对照组外 (P <0 0 5 ) ,其他指标组间无显著性差异 ;再灌注 12 0min时 ,与对照组相比 ,安氟醚预处理组ALT、AST、LDH、ALP及GGT活性明显降低 (P <0 0 5 ) ,异氟醚组各肝功能指标水平均明显降低(P <0 0 5 )。肝脏组织病理学损害 ,安氟醚和异氟醚预处理组明显轻于对照组。结论 安氟醚和异氟醚预处理对肝脏缺血 再灌注损害有一定的保护作用。
Objective To study the effects of the preconditioning with enflurane and isoflurane on hepatic ischemia and reperfusion injury Methods Eighteen rabbits were randomly allocated to receiving no pretrearment (control group, n=6),preconditioning with 1 68% enflurane (PE group,n=6),or preconditioning with 1 15% isoflurane(PI group,n=6), respectively, before undergoing 45 min of hepatic arterial and portal venous occlusions followed by 120 min of reperfusion The arterial blood samples were taken before the occlusion ,10 min and 120 min following reperfusion ,to measure the activities of serum alanine transminase (ALT), aspartate transminase (AST), lactic dehydrogenase (LDH), alkaline phosphatase (ALP) and gama glutaminase(GGT) using auto biochemical analyzer The liver histopathology were observed under light microscope Results Compared with those in control group , in PI and PE group the activities of serum ALT, AST, LDH, ALP and GGT were kept unchanged significantly before the ischemia (P>0 05); the increased degree of only AST activity reduced in PE group(P<0 05), others remained unchanged markedly in PI and PE group (P<0 05) 10 min following reperfusion; the increased degrees of all activities above reduced obviously in PI and PE group (P<0 05)120 min following reperfusion The injury degrees of liver histopathology in PI and PE groups were much less than that in control group Conclusions Preconditoning with enflurane and isoflurane can protect liver from ischemia and reperfusion injury to some extent
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第4期223-225,共3页
Chinese Journal of Anesthesiology
