摘要
【目的】建立光皮桦叶片高效再生体系,为光皮桦的良种培育及遗传转化研究提供依据。【方法】以光皮桦无菌叶片为外殖体,分别用含0.6mg/L TDZ的MS、WPM和1/2MS培养基进行不定芽诱导分化,筛选最佳基本培养基;向筛选出的最佳基本培养基中添加0.05mg/L NAA及不同质量浓度(0.1,0.2,0.4,0.6,0.8,1.0,1.2mg/L)TDZ的激素组合组成不定芽分化培养基,用于诱导不定芽分化,筛选最佳不定芽诱导分化培养基;以1/2MS培养基为基本培养基,分别附加0,0.05,0.1,0.5,1.0,2.0mg/L的IBA或0.5,1.0,2.0,3.0mg/L的NAA组成生根培养基,用于再生苗生根,筛选最佳生根培养基,建立光皮桦叶片再生体系。【结果】光皮桦无菌叶片诱导不定芽的最适基本培养基为MS培养基,其诱导的不定芽最多,且芽生长状况良好,颜色深绿;最适不定芽诱导分化培养基为:MS+TDZ 1.0mg/L+NAA 0.05mg/L+蔗糖30g/L+琼脂5.5g/L,在该培养基上叶片丛生芽诱导率可达到80.00%,平均不定芽数高达12.00个,且芽生长健壮,呈深绿色;最适再生苗生根培养基为:1/2MS+IBA 1.0mg/L+蔗糖20g/L+琼脂5.5g/L,在该培养基上再生苗的生根率达100%,平均根数为14.5个,平均根长为11cm,根较粗壮,基部无愈伤组织,且产生了很多毛细根。【结论】建立了光皮桦无菌苗叶片的高效再生体系。
【Objective】 The regeneration system of Betula luminifera leaf-explants was established and provide scientific reference for the gene transformation and culture of Betula luminifera.【Method】 The optimal basic medium with MS,WPM and 1/2MS mediums containing 0.6 mg/L TDZ was screened using B.luminifera leaf-explants and the optimal basic medium of shoot differentiation with 0.05 mg/L NAA and TDZ of different concentrations（0.1,0.2,0.4,0.6,0.8,1.0,1.2 mg/L）.Take 1/2MS as basic medium,different concentration IBA（0,0.05,0.1,0.5,1.0,2.0 mg/L） or NAA（0.5,1.0,2.0,3.0 mg/L） was added to screen the optimal root differentiation medium.The regeneration system of B.luminifera leaf-explants was established.【Result】 The buds grew well with,dark green on MS medium.The suitable differentiation medium was MS＋TDZ 1.0 mg/L＋NAA 0.05 mg/L＋Suc.30 g/L＋Agar 5.5 g/L,on which the inducing rate of the adventitious shoot was 80.00% and the average buds were 12.00,and the buds grew well,dark green.The inducing rate of the root was 100%,the average roots 14.5,and the length 11 cm,the root stout,base without callus,and produced a lot of hair root when the adventitious shoots were cultured on medium of 1/2MS＋IBA 1.0 mg/L＋ Suc.20 g/L＋Agar 5.5 g/L.【Conclusion】 The regeneration system of B.luminifera leaf-explants was established.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2012年第4期61-67,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家自然科学基金项目(30600484)
浙江省科技计划项目(2008C002-1
Y200907683)
关键词
光皮桦
叶片外植体
再生体系
Betula luminifera H.Winkl； leaf-explants； regeneration system；
