摘要
以无菌条件下培养的山梨组培苗叶片为外植体建立叶片再生体系。结果表明:取继代培养约30d的无菌苗叶片为材料,接种在MS+BA5.0mg/L+NAA0.4mg/L培养基上,20～30d后产生不定芽,再生频率48.6%,再生芽数3.83;暗培养20d叶片再生频率最高;远轴面接触培养基的叶片再生频率和再生芽数高于近轴面接触培养基的叶片;叶片基部的再生频率68.1%,显著高于叶片中部和尖部。
The leaves of aseptic plant were obtained through leaf culture in vitro and then were selected as explants, in order to establish leave regeneration systems for Pyrus ussuriensis. The results showed that when leaves subcultured for 30 days were inoculated in MS medium containing BA 5.0 mg/L＋ NAA 0.4 mg/L, adventitious shoots appeared 20---30 days later, with regeneration frequency being 48.6% and bud number being 3.83 per leaf. The highest regeneration frequency of leaves was obtained when leaves were cultured for 20 days in the dark. The regeneration frequency and bud number per leaf were higher when abaxial side was placed downward onto medium than upward. Regeneration frequency of the basal part of leaves was higher than that of the middle part or top part, reaching 68.1%.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2008年第4期472-476,共5页
Journal of Jilin Agricultural University
基金
吉林省科技发展项目(20060224)
关键词
山梨
叶片
再生体系
Pyrus ussuriensis
leaf
regeneration system
