摘要
OH、O_2~■和病区FA可以使猪软骨Ⅱ型胶原蛋白中羟脯氨酸、脯氨酸含量下降,而谷氨酸含量增加;且O_2^(?)和FA浓度越大,羟脯氨酸含量下降越多。OH,O_2^(?)和FA损伤的胶原蛋白的凝胶化程度和速度降低;CD谱表明其构象发生变化,α-螺旋结构含量下降;SDS-PAGE结果表明,胶原蛋白在-OH、O_2^(?)作用下发生降解生成分子量较小的肽段,而在FA作用下未见明显降解。推测FA作为自由基源在大骨节病的发生中起作用。
To clarify the effects of free radical damaged Type Ⅱ collagen on the mineralization process in cartilage, the kinetics of crystal growth of hydroxyapatite in presence of collagenll from pig cartilage, intact and damaged by. OH and fulvic acid from potable water in epidemic region. The experimental results show that the normal Type Ⅱ collagen inhibits the crystal growth, which is characterized by a lower ks value relative to that obtained in the absence of collagen. In case of the damaged collagens, the crystal growth is accelerated. Nevertheless, all the reactions are of second order. The X-ray diffraction analysis and SEM show that in all cases, the solid phases are attributed to hydroxyapatite, but the damaged Type Ⅱ collage causes the apatite of lower crystallinity and higher agglomeration. These results indicate that the abnormal biomineralization in abnormal cartilage matrix might be an important clue in pathological process in Kaschin-Beck disease.
出处
《北京医科大学学报》
CSCD
1989年第4期307-310,共4页
Journal of Peking University(Health Sciences)
基金
"七五"攻关项目75-62-03-07-01专题
关键词
大骨节病
胶原蛋白
自由基
腐植酸
free radical Fulric acid Collagen Mineralization Hydroxyapatite
